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Morphometric and molecular characterization of pro-and anti-inflammatory cytokines roles in the alveolar bone repair process under homeostatic and infectious conditions

Grant number: 09/11496-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2009
Effective date (End): April 30, 2013
Field of knowledge:Biological Sciences - Immunology
Principal Investigator:Gustavo Pompermaier Garlet
Grantee:Andreia Espíndola Vieira
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Bone is a mineralized connective tissue, whose metabolism control is influenced by endocrine and genetic factors and by the RANK/RANKL/OPG system. The bone also shares a variety of regulatory molecules such as cytokines with the immune system, but little is known about the mechanisms involved in the interaction between skeletal and immune system in the bone repair process. The alveolar repair process after tooth extraction, both in normal and infectious conditions (dry socket typically results in the intense inflammatory response and delayed alveolar repair) can be used as an experimental model to clarify the action mechanisms of different cytokines on bone metabolism. The objective of this project is to elucidate the role of cytokines TNF-alfa and IL-10 in alveolar repair in homeostatic and infectious (experimental dry socket) conditions in WT and knockout (KO) C57Bl/6 mice by means of histomorphometric and molecular characterization. WT, p55KO e IL-10KO mice with C57Bl/6 strain background will be separated into control and experimental dry socket groups and them analyzed in different time points (0, 7, 14 and 21 days). After surgery in the experimental group will be induced dry socket by a suspension of purulent secretion, and samples will be submitted to histological processing and quantitative and qualitative histomorphometric microscopic analyses will be performed, as well the molecular analysis by RealTimePCR for quantification of CBFA1/RUNX2 , OCN, ALP, RANKL, OPG, COL-I and VEGF expression. The data will be statistically analyzed and compared between different strains, groups and time points. The joint analysis of these data certainly will contribute to improve the understanding of the alveolar bone repair process, promoting specific knowledge that can cooperate significantly to the development of new strategies for diagnosis, prevention and therapy of bone diseases.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VIEIRA, ANDREIA ESPINDOLA; REPEKE, CARLOS EDUARDO; FERREIRA JUNIOR, SAMUEL DE BARROS; COLAVITE, PRISCILA MARIA; BIGUETTI, CLAUDIA CRISTINA; OLIVEIRA, RODRIGO CARDOSO; ASSIS, GERSON FRANCISCO; TAGA, RUMIO; FAVARO TROMBONE, ANA PAULA; GARLET, GUSTAVO POMPERMAIER. Intramembranous Bone Healing Process Subsequent to Tooth Extraction in Mice: Micro-Computed Tomography, Histomorphometric and Molecular Characterization. PLoS One, v. 10, n. 5 MAY 29 2015. Web of Science Citations: 32.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
VIEIRA, Andreia Espíndola. Morphometric and molecular characterization of the role of pro- and anti-inflammatory cytokines in alveolar bone repair process under homeostatic and infectious conditions. 2013. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru Bauru.

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