Renal and hepatic toxicity of tepoxalin in dogs and cats with isoflurane-induced hypotension

• Grant number: 10/08698-9
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2010
• End date: January 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Adriano Bonfim Carregaro
• Grantee: Adriano Bonfim Carregaro
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The study aims to investigate some acute and subacute renal and hepatic toxicity with tepoxalin administration in dogs and cats with isoflurane-induced hypotension. Eighteen dogs and eighteen cats, weighing 10 and 20kg and 2 and 4kg, respectively, will be used. The study will follow the same model for both species. Animals will be randomly assigned to either the PRE group, which received tepoxalin orally (10mg/kg to dogs and 20mg/kg to cats) two hours before anesthesia, the POST group, which receiving the same dose 30 minutes after anesthesia, and the control group, which only will be anesthetized. For the subacute study, animals in the PRE and POST will also receive tepoxalin every 24 hours over five days. The animals will be induced and maintained with isoflurane anesthesia in vaporization sufficient to mantain MAP between 50-60mmHg, featuring a moderate hypotension condition. They will be ventilated by IPPV (10cm/H2O; I/E 1,5), and FR will be adjusted to keep ETCO2 in the range of 35 - 45 mmHg. Body temperature will be mantained at physiological range of species by thermal mattress. HR, SAP, MAP, DAP, CVP, ETCO2, FR and ETIso will be evaluated at 0, 10, 20 and every 10min to 60min. pH, PaO2, PaCO2, SaO2, HCO3-, DB, Na+, K+ and Ca2 + will be obtained at 0, 30 and 60min. Renal and hepatic functions will be performed by serum levels of BUN, creatinine (Cr), alkaline phosphatase, alanine aminotransferase, c-glutamyltransferase (GGT), total protein and urinary excretion of GGT, Cr, and GGT:Cr ratio at 12h, 24h and seven days after the procedure. Thus, it is expected to verify the safety of the tepoxalin in animals with isoflurane-induced hypotension, and also to show if will occur subacute deleterious effects associated with its use during five days. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES, CARLIZE; CARREGARO, ADRIANO B.; FREITAS, GABRIELLE C.; PADILHA, VANESSA S.; LUKARSEWSKI, RAFAEL; PAIM, FRANCINE C.; LOPES, SONIA T. DA. Effect of tepoxalin on renal function and hepatic enzymes in dogs exposed to hypotension with isoflurane. VETERINARY ANAESTHESIA AND ANALGESIA, v. 41, n. 5, p. 459-467, SEP 2014. (10/08698-9)

FREITAS, GABRIELLE COELHO; CARREGARO, ADRIANO BONFIM; BISETTO, SHAYNE PEDROZO; BARBOSA, ALINE SOARES; FANTINATO NETO, PAULO; ALMEIDA LEITE DELLOVA, DEISE CARLA; LOPES, CARLIZE. Tepoxalin on renal function and liver enzymes in cats exposed to hypotension with isoflurane. Ciência Rural, v. 44, n. 6, p. 1073-1081, JUN 2014. (10/08698-9)