Gene expression analysis, biofilm formation and adherence to Caco-2 cells by Listeria monocytogenes in different conditions found in food

• Grant number: 09/51141-8
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2009
• End date: January 31, 2012
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Elaine Cristina Pereira de Martinis
• Grantee: Elaine Cristina Pereira de Martinis
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Listeria monocytogenes is a bacteria transmitted mainly by food, causing serious infections in immunosuppressed persons and pregnants, because of its ability to survive intracellular. L. monocytogenes control is a challenge, especially in chilled and ready to eat foods, due to be a psychotropic, ubiquitous and relatively resistant to adverse environmental conditions. The biofilms formation by L. monocytogenes is a worrying factor for the food industries, because biofilms undertake the sanitization of surfaces, increasing the risk of contamination. Furthermore, some studies indicate that the capacity of biofilm formation can be correlated with the ability of L. monocytogenes causing disease. L. monocytogenes shows ability to adapt rapidly to different environmental conditions by modulating the expression of their genes. In this project, L. monocytogenes will be cultivated in different conditions found in food and the host. The strains will be studied for assessment of their capacity to form biofilms, in vitro adherence to intestinal epithelial cells and modulation of gene expression (inlA). (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WINKELSTROETER, L. K.; DE MARTINIS, E. C. P.. In vitro protective effect of lactic acid bacteria on Listeria monocytogenes adhesion and invasion of Caco-2 cells. BENEFICIAL MICROBES, v. 6, n. 4, p. 535-542, 2015. (09/51141-8)

WINKELSTROETER, LIZZIANE KRETLI; DE MARTINIS, ELAINE C. P.. Effect of Bacteriocins and Conditions that Mimic Food and Digestive Tract on Biofilm Formation, In Vitro Invasion of Eukaryotic Cells and Internalin Gene Expression by Listeria monocytogenes. PROBIOTICS AND ANTIMICROBIAL PROTEINS, v. 5, n. 3, p. 153-164, SEP 2013. (09/51141-8)

WINKELSTROETER, LIZZIANE KRETLI; TULINI, FABRICIO LUIZ; DE MARTINIS, ELAINE C. P.. Identification of the bacteriocin produced by cheese isolate Lactobacillus paraplantarum FT259 and its potential influence on Listeria monocytogenes biofilm formation. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 64, n. 2, p. 586-592, DEC 2015. (09/51141-8, 11/11983-0)

TULINI, FABRICIO LUIZ; WINKELSTROETER, LIZZIANE KRETLI; DE MARTINIS, ELAINE C. P.. Identification and evaluation of the probiotic potential of Lactobacillus paraplantarum FT259, a bacteriocinogenic strain isolated from Brazilian semi-hard artisanal cheese. Anaerobe, v. 22, p. 57-63, AUG 2013. (09/51141-8, 11/11983-0)