Evaluation of microbial ecology on the presence of Listeria monocytogenes and Salmonella sp. in food processing industries, the formation of multispecies biofilms and control methods

• Grant number: 20/01529-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): September 01, 2020
• Effective date (End): February 26, 2023
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Products of Animal Origin Inspection
• Principal Investigator: Jose Paes de Almeida Nogueira Pinto
• Grantee: Leonardo Ereno Tadielo
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Brazil occupies ample space in the world food scene, the expansion of these markets, allied to the export rates, makes sanitary conditions essential to guarantee food safety and commercial stability. Among the factors that affect food safety, pathogenic microorganisms deserve to be highlighted, as they are related to the occurrence of diseases. Listeria monocytogenes and Salmonella sp. are ubiquitous, and are therefore found in numerous animal products and surfaces of equipment and utensils for industrial use. Such characteristics may be related to the ability to adhere and form biofilms on biotic or abiotic surfaces, which gives bacterial cells protection, adaptability and resistance to technological processes. Thus, understanding the Microbial Ecology associated with the presence of L. monocytogenes and Salmonella sp. isolated after the pre-operational hygiene programs of the industries, allows the diagnosis of the same ecological niches of survival, and the knowledge of the accompanying Microbial Ecology allows to show if there is synergism, competition, antagonism or non-interference in the development of one species due to another. As well, through the dynamics of adhesion and formation of biofilms in vitro, it is possible to understand the participation of accompanying Microbial Ecology in the survival of pathogenic microorganisms and, with this, validate tests for prevention and elimination of biofilms with greater accuracy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TADIELO, LEONARDO ERENO; DOS SANTOS, EMANOELLI APARECIDA RODRIGUES; POSSEBON, FABIO SOSSAI; SCHMIEDT, JHENNIFER ARRUDA; ORISIO, PAULO HENRIQUE SILVA; JULIANO, LARA CRISTINA BASTOS; CERQUEIRA-CEZAR, CAMILA KOUTSODONTIS; PINTO, JOSE PAES DE ALMEIDA NOGUEIRA; PEREIRA, JULIANO GONCALVES; BERSOT, LUCIANO DOS SANTOS. Preoperational cleaning processes interfere with microbial ecology and presence of Listeria monocytogenes and Salmonella spp. on food conveyor belts of a poultry slaughterhouse in Brazil. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 184, p. 10-pg., 2023-07-15. (20/01529-9)

TADIELO, LEONARDO ERENO; DOS SANTOS, EMANOELLI APARECIDA RODRIGUES; POSSEBON, FABIO SOSSAI; SCHMIEDT, JHENNIFER ARRUDA; JULIANO, LARA CRISTINA BASTOS; CERQUEIRA-CEZAR, CAMILA KOUTSODONTIS; DE OLIVEIRA, JANAINA PRIETO; SAMPAIO, ARYELE NUNES DA CRUZ ENCIDE; MELO, PATRICIA REGINA LOPES; CARON, EVELYN FERNANDA FLORES; et al. Characterization of microbial ecology, Listeria monocytogenes, and Salmonella sp. on equipment and utensil surfaces in Brazilian poultry, pork, and industries. Food Research International, v. 173, p. 12-pg., 2023-11-01. (20/01529-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

TADIELO, Leonardo Ereno. Evaluation of microbial ecology, Listeria monocytogenes and Salmonella sp. in food processing industries, multispecies biofilm formation and control methods. 2023. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.