Mass spectrometry accelerated the secondary metabolites profiling in microbial extracts from actinomycetes

• Grant number: 09/51145-3
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2009
• End date: September 30, 2011
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Luiz Alberto Beraldo de Moraes
• Grantee: Luiz Alberto Beraldo de Moraes
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

A major potential of NPs is the fact that many natural product resources are largely unexplored, and many environmental samples for isolation of interesting microorganisms have often been collected without a defined strategy. Microorganisms, in particular the actinomycetes are known to produce a vast number of bioactive secondary metabolites of interesting pharmaceuticals and agrochemical industry. Rapid profiling techniques have been desired for many years, which ultimately may determine all metabolites produced by a microorganism. In the current age of omics this quest is now a part of what is known as the metabolomics, which aims to detect all small metabolites in a cell or organism. This means that direct metabolite profiling techniques such as direct infusion mass spectrometry or LC-MS/MS can easily be used for chemotyping/metabolomics of strains from culture collections. This context, ours main goal is to applied direct-infusion electro spray mass spectrometry (ES-MS) technique to identify the effects of different growth conditions on expression of secondary metabolites by actinomycetes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CREVELIN, EDUARDO J.; CROTTI, ANTONIO E. M.; ZUCCHI, TIAGO D.; MELO, ITAMAR S.; MORAES, LUIZ A. B.. Dereplication of Streptomyces sp AMC 23 polyether ionophore antibiotics by accurate-mass electrospray tandem mass spectrometry. Journal of Mass Spectrometry, v. 49, n. 11, p. 1117-1126, NOV 2014. (13/20094-0, 09/51145-3)