Biological interaction bioactive polymeric membranes in rabbit tibia: morphometric, histological and immunohistochemistry analysis

• Grant number: 10/01468-8
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2010
• End date: March 31, 2012
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Cassio Ricardo Auada Ferrigno
• Grantee: Cassio Ricardo Auada Ferrigno
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Walter Israel Rojas Cabrera

Abstract

The development of graft material, membranes and tridimensional matrixes, based on absorbable and biocompatible biomaterials, as well as their clinical applications and influence on the tissue regeneration process, has shown the need for studies on synthetic and natural polymers synthesis, within the context of tissue engineering. There are various membranes on the market among which there are the osteoinductors, osteoconductors and osteogenics ones. Each of these subtypes, as well as its brands, has its limitations, such as low bioactivity, need for antibiotics, need for a second surgical intervention for the removal of the membrane (for the non-absorbable) and little acting time of the absorbable membranes (around 30 to 40 days). Due to these limitations, it's necessary to develop new biomaterials that form bone matrix with biological characteristics and that are mechanically adequate, susceptible to hydrolysis or enzymatic degradation in non-toxic substances, in a time compatible with tissue formation. Morphometric, histological and immunohistochemistry analysis will be made, for the comparison of the interaction of polymeric bioactive membranes, of three types of membranes implanted in rabbit tibias. Absorption degree of the membrane, inflammation and reparation (osteogenesis and/or fibrogenesis). These aspects will be analyzed in a qualitative and quantitative form, with morphometric tests to evaluate celularity, amount of collagen fibers and osteoid formation. The data will be compared with non-parametric statistical tests. The project may support the use of the biomaterial in pet and human research. As the theme involves the integration of different fields, such as biology, medicine, odontology, engineering, chemistry and physics, this study will also be used for studies in these related areas. The clinical situations where great bone loss are frequent and the use of biomaterials may be important in these surgical challenges. Generally therapeutic osteotomies / ostectomies may cause "gaps" that are slowly filled by bone, and the biomaterial may accelerate this healing process. (AU)