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Fertility and uterine inflammatory response in bovine post artificial insemination using semen evaluated by fluorescent probes associations

Abstract

The objectives of this work are verify the fertility rate and uterine inflammatory response in bovine after artificial insemination (AI) with semen evaluated by fluorescent probes associations to assess simultaneously sperm plasma and acrosomal membranes integrity and mitochondrial function and evaluate the methods to estimate uterine inflammatory response. Will be realized 3 experiments. The experiment 1 will be performed to study the uterine homodynamic in bovine after AI by color Doppler ultrasound, for this 10 cows will be synchronized (estrous and ovulation) and divided in 2 groups: not inseminated group (control, n=5) and submitted to AI group (n=5). In the experiment 2 will be compared the techniques of uterine inflammatory response evaluation in bovine by saline solution infusion (UI) and by swab with cytobrush (CB), considering quantity and quality of the sample and theirs effects on uterine environment by color Doppler ultrasound and endometrial biopsy as controls, will be utilized 10 cows divided in 2 groups: submitted to CB group (n=5) and submitted to a UI group (n=5). To experiment 3 will be utilized 360 cows submitted to a timed AI protocol, divided in 3 groups with regard to percentage of cells showing plasma membrane and acrosomal integrity and mitochondrial function (PIAIC), assessed by fluorescent probes (PI, FITC-PSA and JC-1): 10 to 20% (n=120), 30 to 40% (n=120) and 50 to 60% PIAIC sperm/straw (n=120). The females will be monitored about uterine inflammatory response post Al. Pregnancy diagnostic will be performed 30 days after AI. The data from experiments 1 and 2 will submit to variance analysis, and that from experiment 3 will analyzed by X2 test and linear regression. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
THOME, HELDER ESTEVES; DE ARRUDA, RUBENS PAES; MARTINS DE OLIVEIRA, BRUNA MARCELE; MATURANA FILHO, MILTON; DE OLIVEIRA, GUILHERME CAIN; GUIMARAES, CARINA DE FATIMA; DE CARVALHO BALIEIRO, JULIO CESAR; AZEDO, MILTON RICARDO; POGLIANI, FABIO CELIDONIO; CARVALHO CELEGHINI, ENEIVA CARLA. Uterine lavage is efficient to recover endometrial cytology sample and does not interfere with fertility rate after artificial insemination in cows. Theriogenology, v. 85, n. 9, p. 1549-1554, JUN 2016. Web of Science Citations: 2.
OLIVEIRA, BRUNA MARCELE; ARRUDA, RUBENS PAES; THOME, HELDER ESTEVES; MATURANA FILHO, MILTON; OLIVEIRA, GUILHERME; GUIMARAES, CARINA; NICHI, MARCILIO; SILVA, LUCIANO ANDRADE; CARVALHO CELEGHINI, ENEIVA CARLA. Fertility and uterine hemodynamic in cows after artificial insemination with semen assessed by fluorescent probes. Theriogenology, v. 82, n. 5, p. 767-772, SEP 15 2014. Web of Science Citations: 7.
MARTINS DE OLIVEIRA, BRUNA MARCELE; DE ARRUDA, RUBENS PAES; THOME, HELDER ESTEVES; MATURANA FILHO, MILTON; DE OLIVEIRA, GUILHERME CAIN; GUIMARAES, CARINA DE FATIMA; SILVA, LUCIANO ANDRADE; NICHI, MARCILIO; CARVALHO CELEGHINI, ENEIVA CARLA. Artificial insemination causes uterine hemodynamic alterations in suckled beef cows subjected to an ovulation synchronization program. LIVESTOCK SCIENCE, v. 167, p. 449-454, SEP 2014. Web of Science Citations: 4.

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