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Matrix metalloproteinase modulation in contat with bonding agents used in dentistry, subcutaneous tissue evaluation in mice and as indirect pulp capping material in dogs teeth

Grant number: 09/54573-6
Support type:Regular Research Grants
Duration: February 01, 2010 - January 31, 2012
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Léa Assed Bezerra da Silva
Grantee:Léa Assed Bezerra da Silva
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Bonding agents are widely used in Dentistry, although the compatibility of these materials with pulp tissue and the mechanisms involved in tissue remodeling are not known. Therefore, the aim of this research will be the evaluation of the biocompatibility of resin-based and glass ionomer-based bonding agents in the subcutaneous tissue of mice and as indirect pulp capping agent in dogs teeth. Then, tissue remodeling will be investigated to determinate the presence and activity of matrix metalloproteinases (MMPs) in the process. Test material will be implanted in the subcutaneous tissue of Balb/C mice and in dental cavities prepared in dogs teeth. A glass ionomer-based Ketac Cem Easy Mix and resin-based bonding agents Multilink Automix and Rely X Unicem will be evaluated. Following experimental time, the animals will be euthanized and the tissues will be prepared for routine histology. Tissue compatibility will be investigated to determinate the degree of fibrosis induced by the material, as well as the intensity of the inflammatory infiltrate and the presence of reactionary / reparative dentin formation. To further investigate the possible mechanisms involved in the response, the presence and activity of matrix metalloproteinases will be evaluated by means of in situ zymography and immunohistochemistry. Data obtained will be submitted to statistical analysis according to the pattern of distribution of the samples using one way ANOVA followed by Tukey test, Mann-Whitney or Exact Fisher tests (a= 0.05). (AU)