Analysis of non-steroid antiinflammatory effects in the c-Fos expression during experimental tooth movement

• Grant number: 09/02468-4
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2009
• End date: January 31, 2012
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Maria José Alves da Rocha
• Grantee: Maria José Alves da Rocha
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs) have been used for pain relief in orthodontics, but clinical studies reported that they may reduce tooth movement (TM). By otherside, TM seems to activate brain structures related to nociception, but the effects of NSAIDs in this activation have not been studied yet. We will analyse the effect of short-term treatment with acetaminophen or celecoxib in the separation of rat superior incisors, in the periodontal structures as well as in neuronal activation of the spinal trigeminal nucleus (SpV), following tooth movement. After determinating the best force to be applied to induce tooth movement without causing palate expansion, thirty rats (400-420g) were pretreated through oral gavage (1 ml/dose) with acetaminophen (200mg/kg), celecoxib (50mg/kg) or vehicle (Carboxymethylcellulose 0.4%). After 30 min, they will receive an activated orthodontic appliance for tooth movement. In controls, this appliance will be immediately removed after its introduction. Rats will receive ground food, and every 12 h, one of the drugs or vehicle. After 48 or 72 hours, they will be anesthetized, the maxilla will be radiographed, and they will be perfused with 4% paraformaldehyde. Brains and maxilla will be removed and further processed for Fos immunohistochemistry and analysis of periodontal structures. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

STABILE, A. C.; STUANI, M. B. S.; LEITE-PANISSI, C. R. A.; ROCHA, M. J. A.. Effects of short-term acetaminophen and celecoxib treatment on orthodontic tooth movement and neuronal activation in rat. Brain Research Bulletin, v. 79, n. 6, p. 396-401, AUG 14 2009. (09/02468-4)