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Interactomic analysis of hexokinase-VDAC-ANT complex in rat, bovine and chicken neuronal cells


The voltage dependent anion channel is the most abundant protein of outer mitochondrial membrane. VDAC controls the metabolite exchange through this membrane and the apoptosis machinery. Interactomics is the study of protein complexes, their interactions and the consequences of these interactions. In our case we will study the interactomics of VDAC and other proteins inside the neuron. Other purpose is to understand the stoichiometry of the complex hexokinase-VDAC-ANT, in chicken, rat and bovine brain. Previous studies from our laboratory have shown a differential expression of VDAC 1 and VDAC2 between these brains. To address this problem, in the present study, we wish to see if the there are differences in the interactions of these proteins in the different brains. Chicken brain mitochondria show the highest VDCA1 expression and the lowest of VDAC2. Other purpose is to see if there is a VDAC1 and VDAC2 differential expression in avian neuronal cells submitted to apoptosis by staurosporin. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROSA, JOSE CESAR; CESAR, MARCELO DE CERQUEIRA. Role of Hexokinase and VDAC in Neurological Disorders. CURRENT MOLECULAR PHARMACOLOGY, v. 9, n. 4, p. 320-331, 2016. Web of Science Citations: 7.
CREPALDI, CARLA ROSSINI; MARIANO VITALE, PHELIPE AUGUSTO; TESCH, ANDREA CRISTINA; LAURE, HELEN JULIE; ROSA, JOSE CESAR; CESAR, MARCELO DE CERQUEIRA. Application of 2D BN/SDS-PAGE coupled with mass spectrometry for identification of VDAC-associated protein complexes related to mitochondrial binding sites for type I brain hexokinase. MITOCHONDRION, v. 13, n. 6, p. 823-830, NOV 2013. Web of Science Citations: 2.
CARLA ROSSINI CREPALDI; GIOVANA KREMPEL FONSECA MERIGHE; HÉLEN JULIE LAURE; JOSÉ CÉSAR ROSA; FLÁVIO VIEIRA MEIRELLES; MARCELO DE CERQUEIRA CÉSAR. Isolamento e cultivo de neurônios e neuroesferas de córtex cerebral aviar. Pesquisa Veterinária Brasileira, v. 33, p. -, Dez. 2013.
PHELIPE AUGUSTO MARIANO VITALE; CARLA ROSSINI CREPALDI; ANDRÉA CRISTINA TESCH; RICARDO DE ALBUQUERQUE; MARCELO DE CERQUEIRA CÉSAR. Purificação e caracterização da VDAC de mitocôndrias corticais aviares: identificação de modificações pós-traducionais nas porinas neuronais murinas e aviares. Pesquisa Veterinária Brasileira, v. 32, n. 12, p. -, Dez. 2012.

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