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Molecular characterization and signaling pathways of histological special types of the breast

Grant number: 10/00815-6
Support type:Regular Research Grants
Duration: April 01, 2010 - March 31, 2012
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Luiz Carlos Zeferino
Grantee:Luiz Carlos Zeferino
Home Institution: Centro de Atenção Integral à Saúde da Mulher (CAISM). Hospital da Mulher Professor Doutor José Aristodemo Pinotti. Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Assoc. researchers: César Augusto Alvarenga ; Fernando Carlos de Lander Schmitt ; Rozany Mucha Dufloth


Invasive breast carcinomas represent a heterogeneous group of tumors that show a wide variation in terms of their clinical presentation, behavior and morphology and are described at least 18 different histologic types of breast carcinomas by the World Health Organization (WHO). However, there are few available studies on the molecular profiles and characterization of intracellular signaling pathways of the special histologic subtypes that together represent the vast majority of breast tumors. This study may help to identify prognostic markers of therapeutic response. OBJECTIVE: To characterize the molecular profile of specific histological subtypes of breast cancer using the following immunohistochemical markers: estrogen receptor, progesterone receptor, HER2, CK5, P-cadherin, EGFR, MIB-1 and p53, as well as markers of pathways signaling intracelulares. MATERIAIS AND METHODS: Will be selected, consecutive paraffin blocks of women with breast cancer and whose histological diagnoses were mucinous carcinoma, tubular, micropapilífero, intracystic papillary, apocrine, and metaplastic in three Institutions. Blocks will be extracted donors cylinders 2 mm in diameter and deposited in the recipient paraffin blocks using Tissue Microarray builder ab1802 (Abcam ®, Cambridge, UK). These cuts will be made to search for molecular markers of protein expression and signaling pathways for breast carcinomas. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE BECA, FRANCISCO FERRO; CAETANO, PEDRO; GERHARD, RENE; ALVARENGA, CESAR AUGUSTO; GOMES, MADALENA; PAREDES, JOANA; SCHMITT, FERNANDO. Cancer stem cells markers CD44, CD24 and ALDH1 in breast cancer special histological types. Journal of Clinical Pathology, v. 66, n. 3, p. 187-191, MAR 2013. Web of Science Citations: 75.
ALVARENGA, CESAR AUGUSTO; PARAVIDINO, PAULA ITAGYBA; ALVARENGA, MARCELO; GOMES, MADALENA; DUFLOTH, ROZANY; ZEFERINO, LUIZ CARLOS; VASSALLO, JOSE; SCHMITT, FERNANDO C. Reappraisal of immunohistochemical profiling of special histological types of breast carcinomas: a study of 121 cases of eight different subtypes. Journal of Clinical Pathology, v. 65, n. 12, p. 1066-1071, DEC 2012. Web of Science Citations: 8.
ALVARENGA, CESAR A.; PARAVIDINO, PAULA I.; ALVARENGA, MARCELO; DUFLOTH, ROZANY; GOMES, MADALENA; ZEFERINO, LUIZ C.; SCHMITT, FERNANDO. Expression of CK19 in invasive breast carcinomas of special histological types: implications for the use of one-step nucleic acid amplification. Journal of Clinical Pathology, v. 64, n. 6, p. 493-497, JUN 2011. Web of Science Citations: 25.

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