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Tissue, saliva and blood comparison of human papillomavirus (HPV) detection in patiens with squamous cell carcinoma and controls


In Brazil, the estimative of oral cavity cancer incidence for 2010 is 10,330 new cases for men and 3,790 for women (INCA, 2009). The squamous cell carcinoma (SCC) it corresponds the approximately 90% of all carcinomas (Luo et al., 2007). Human papillomavirus (HPV) it has been related with head and neck SCC etiology, about 60% of these carcinomas can be HPV positive (Kreimer et al., 2005). HPV has also been detected in the serum and saliva of patients with cervical carcinoma with rates that vary from 7 to 45% (Ho et al., 2005). In the head and neck regions, the HPV was detected in 45,6% of the SCC patients by saliva analysis (Zhao et al., 2005). The aim of this study is to detect the HPV presence in genetic material obtained of biopsies, oral swabs, saliva and blood of SCC patients and controls. It will be made an statistical analysis of genetic material of 40 SCC patients and 30 controls patients without carcinoma. The DNA extraction of all specimens will be accomplished using the phenol-chloroform method. The isolated DNA will be submitted PCR for beta-globin to confirm the DNA presence and integrity, after new reactions will be accomplished for HPV DNA detection. The results will be submitted to statistical analyses to detect the agreement of the viral DNA detection in the several analyzed genetic materials. (AU)