P53 and C-myc genic expression of the ocular adnexa tumors in dogs

• Grant number: 08/00429-9
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2008
• End date: March 31, 2010
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Alexandre Lima de Andrade
• Grantee: Alexandre Lima de Andrade
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The occurrence of ocular tumors in small animals is low, however, the tumors of epithilial origin is one of the most, been the squamous cell carcinoma of the most malignant tumors, and with an incidence ranging from three to 20%. The eyelids, conjunctival and cornea are the structures most frequently affected. Among the various technologies used to explore the gene expression in cancer, the PCR and RT-PCR techniques have demonstrated to be useful for the determination of the expression of oncogenes involved in the genesis of these tumors. The recent growth of research in molecular biology as an important tool in the study of cancer cases has identified a wide range of the number of markers that can form a basis for the stratification of various types of cancers in humans and dogs, assisting in the diagnosis, therapy and prognosis. The p53 gene, also named "guardian of the genome" is located in the man, on chromosome 17 and is the most common target for genetic changes in tumors in humans and animals. The C-myc oncogene has been identified as central in carcinogenesis in several tumors in humans. Recent studies have shown participation, direct and indirect protein C-myc with action in the regulation of cell cycle, differentiation, metabolism, cell growth, apoptosis, genomic instability, immortality, angiogenesis and sensitivity to chemotherapy drugs. The purpose of this study will be to identify the gene expression of these oncogenes in escamous cell carcinomas of the eye in dogs. Then, twelve paraffin-embedded tissues, previously classified histologically, will be analyse. The PCR and RT-PCR techniques will be use to identify the presence of these genes and their expression, respectively. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES, RODRIGO ANTONIO; CARDOSO, TEREZA CRISTINA; RUI LUVIZOTTO, MARIA CECILIA; DE ANDRADE, ALEXANDRE LIMA. Occurrence and expression of p53 suppressor gene and c-Myc oncogene in dog eyelid tumors. VETERINARY OPHTHALMOLOGY, v. 13, n. 2, p. 69-75, MAR 2010. (08/00429-9)