Prognostic value of ATM genic and protein expression in bitches with breast cancers

• Grant number: 11/10279-7
• Support type: Regular Research Grants
• Duration: August 01, 2011 - July 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal researcher: Renee Laufer Amorim
• Grantee: Renee Laufer Amorim
• Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Assoc. researchers: Andrigo Barboza de Nardi ; Erika Maria Terra ; Mirela Tinucci Costa ; Talita Mariana Morata Raposo Ferreira

Abstract

Breast cancer is the main tumor that affects bitches and their biological behavior is similar to women, so bitches can be an excellent comparative model in the study of the carcinogenic process in this kind of neoplasia. Metastasis is a common cancer consequence it can lead to death. Identification of tumor markers can be a useful tool in evaluating prognostic and predictive factors. The aim of this study is to evaluate ATM gene and protein expression and other markers such as TP53 e VEGF by RT-PCR and immunohistochemistry methods, respectively. TP53 and VEGF are in the same pathway as ATM and they are incriminated in metastatic process. These markers seem to have an important interaction in women breast cancer, therefore the study of this association may provide a new perspective on the treatment and prognosis of breast tumors in female dogs. We are going to use 40 canine mammary glands, divided into four groups of 10 animals each: G1 = normal breasts, with no history of cancer, G2 = benign breast neoplasm, G3 = malignant breast tumorswithout evidence of metastasis and G4= malignant breast cancer and with metastasis. The results will be evaluated for the level gene and protein expression of these genes in tissue, their relationship with biological behavior and histological type of tumor, presentation of recurrence or metastasis by the method of analysis of variance. Therefore, we hope to better understand the involvement of these markers in the carciongenic process and development of metastasis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAPOSO-FERREIRA, T. M. M.; BUENO, R. C.; TERRA, E. M.; AVANTE, M. L.; TINUCCI-COSTA, M.; CARVALHO, M.; CASSALI, G. D.; LINDE, S. D.; ROGATTO, S. R.; LAUFER-AMORIM, R. Downregulation of ATM Gene and Protein Expression in Canine Mammary Tumors. VETERINARY PATHOLOGY, v. 53, n. 6, p. 1154-1159, NOV 2016. Web of Science Citations: 1.

RAPOSO-FERREIRA, TALITA M. M.; SALVADOR, ROSANA C. L.; TERRA, ERIKA M.; FERREIRA, JUAREZ H.; VECHETTI-JUNIOR, IVAN JOSE; TINUCCI-COSTA, MIRELA; ROGATTO, SILVIA R.; LAUFER-AMORIM, RENEE. Evaluation of vascular endothelial growth factor gene and protein expression in canine metastatic mammary carcinomas. MICROSCOPY RESEARCH AND TECHNIQUE, v. 79, n. 11, p. 1097-1104, NOV 2016. Web of Science Citations: 5.

MORATA RAPOSO-FERREIRA, TALITA MARIANA; VARALLO, GIOVANNA ROSSI; CALAZANS, SABRYNA GOUVEIA; JARK, PAULO CESAR; LINO SALVADOR, ROSANA DA CRUZ; TINUCCI-COSTA, MIRELA; DE NARDI, ANDRIGO BARBOZA; CASSALI, GEOVANNI DANTAS; LAUFER-AMORIM, RENEE. Paraneoplastic Hypercalcemia Secondary to Canine Mammary Tumors. Acta Scientiae Veterinariae, v. 44, SEP 6 2016. Web of Science Citations: 0.