Project Uçá III - Genotoxic impact on population of uçá-crab, Ucides cordatus (Linnaeus, 1763) (Crustacea, Brachyura, Ucididae): evaluation and correlation with of heavy metal concentration in five mangroves of the São Paulo State

• Grant number: 09/14725-1
• Support type: Regular Research Grants
• Duration: February 01, 2010 - July 31, 2012
• Field of knowledge: Biological Sciences - Zoology - Taxonomy of Recent Groups
• Principal Investigator: Marcelo Antonio Amaro Pinheiro
• Grantee: Marcelo Antonio Amaro Pinheiro
• Home Institution: Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil

Abstract

This study aims to evaluate contamination of water, sediment, vegetation and the uçá-crab by heavy metals in five mangroves of the São Paulo State, and to analyze the genotoxic impact on populations of this crustacean, based on the frequency of micronuclei cells. Mangroves are Permanent Preservation Areas (PPAs) and nurseries for many species, including the uçá-crab, which is widely marketed and consumed by humans in coastal regions. Five mangroves areas will be evaluated in São Paulo State (Cananéia, Iguape, Juréia, Cubatão and São Vicente), which was previously selected according to their level of pollution. Each mangrove will be represented by three sampling sub-areas. Sediments, tree leaves (Rhizophora mangle), corporeal structures from uçá-crab (Ucides cordatus) and water from crustacean burrows will be sampled for the quantification of six heavy metals (Cd, Cu, Pb, Cr, Mn and Hg) by atomic absorption spectrophotometry. The haemolymph of five crabs caught/subarea (n=75) will be used for the preparation of three slides/specimen and after staining they will be used to quantify the number of micronuclei cells (MCs) under microscope (1000X). Data obtained by quantification of MCs as well as heavy metal levels will be submitted to ANOVA and Nested ANOVA, respectively, which averages will be compared by Tukey's test (5%). The results obtained will assess the conservation status of mangroves and quality of natural stocks of U. cordatus, guiding actions to be leaded by government agencies for their management. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ALMEIDA DUARTE, LUIS FELIPE; DE SOUZA, CAROLINE ARAUJO; SEABRA PEREIRA, CAMILO DIAS; AMARO PINHEIRO, MARCELO ANTONIO. Metal toxicity assessment by sentinel species of mangroves: In situ case study integrating chemical and biomarkers analyses. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 145, p. 367-376, NOV 2017. Web of Science Citations: 7.

DA SILVA BANCI, KARINA RODRIGUES; MORI, GUSTAVO MARUYAMA; DE OLIVEIRA, MARCOS ANTONIO; PAGANELLI, FERNANDA LAROZA; PEREIRA, MARIANA RANGEL; AMARO PINHEIRO, MARCELO ANTONIO. Can environmental pollution by metals change genetic diversity? Ucides cordatus (Linnaeus, 1763) as a study case in Southeastern Brazilian mangroves. Marine Pollution Bulletin, v. 116, n. 1-2, p. 440-447, MAR 15 2017. Web of Science Citations: 6.

WUNDERLICH, ALISON CARLOS; CARMONA-SUAREZ, CARLOS ALBERTO; AMARO PINHEIRO, MARCELO ANTONIO. NEW RECORDS OF THE ISOPOD Leidya distorta INFESTING THE MANGROVE CRAB Ucides cordatus FROM SOUTH AMERICA. BOLETIM DO INSTITUTO DE PESCA, v. 43, n. 2, p. 283-290, 2017. Web of Science Citations: 0.

DE ALMEIDA DUARTE, LUIS FELIPE; DE SOUZA, CAROLINE ARAUJO; NOBRE, CAIO RODRIGUES; SEABRA PEREIRA, CARRILLO DIAS; AMARO PINHEIRO, MARCELO ANTONIO. Multi-level biological responses in Ucides cordatus (Linnaeus, 1763) (Brachyura, Ucididae) as indicators of conservation status in mangrove areas from the western atlantic. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 133, p. 176-187, NOV 2016. Web of Science Citations: 7.

LUIS FELIPE DE ALMEIDA DUARTE; RICARDO SANTOS DURAN; JOCEMAR TOMASINO MENDONÇA; MARCELO ANTONIO AMARO PINHEIRO. Fishery of the Uçá Crab Ucides Cordatus (Linnaeus, 1763) in a Mangrove Area in Cananéia, State of São Paulo, Brazil: Fishery Performance, Exploitation Patterns and Factors Affecting the Catches. Brazilian Journal of Oceanography, v. 62, n. 3, p. -, Set. 2014.

DE ALMEIDA DUARTE, LUIS FELIPE; DURAN, RICARDO SANTOS; MENDONCA, JOCEMAR TOMASINO; AMARO PINHEIRO, MARCELO ANTONIO. FISHERY OF THE UCA CRAB Ucides cordatus (LINNAEUS, 1763) IN A MANGROVE AREA IN CANANEIA, STATE OF SAO PAULO, BRAZIL: FISHERY PERFORMANCE, EXPLOITATION PATTERNS AND FACTORS AFFECTING THE CATCHES. Brazilian Journal of Oceanography, v. 62, n. 3, p. 187-199, Set. 2014. Web of Science Citations: 8.

PINHEIRO, M. A. A.; DUARTE, L. F. A.; TOLEDO, T. R.; ADAM, M. L.; TORRES, R. A. Habitat monitoring and genotoxicity in Ucides cordatus (Crustacea: Ucididae), as tools to manage a mangrove reserve in southeastern Brazil. ENVIRONMENTAL MONITORING AND ASSESSMENT, v. 185, n. 10, p. 8273-8285, OCT 2013. Web of Science Citations: 21.

AMARO PINHEIRO, MARCELO ANTONIO; GANDARA E SILVA, PABLO PENA; DE ALMEIDA DUARTE, LUIS FELIPE; ALMEIDA, ALAOR APARECIDO; ZANOTTO, FLAVIA PINHEIRO. Accumulation of six metals in the mangrove crab Ucides cordatus (Crustacea: Ucididae) and its food source, the red mangrove Rhizophora mangle (Angiosperma: Rhizophoraceae). ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 81, p. 114-121, JUL 1 2012. Web of Science Citations: 35.