Automatic DNA sequencer update of INFAR Multi-user Laboratory, UNIFESP-EPM

• Grant number: 09/05405-3
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2009
• End date: July 31, 2011
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Aparecida Sadae Tanaka
• Grantee: Aparecida Sadae Tanaka
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The multi-user of Automatic DNA Sequencing Unit in the Pharmacology and Molecular Biology Institute (INFAR) of UNIFESP-EPM has been working since March, 2001, being financial supported by FAPESP multi-user Project (Proc. No. 1999/12347-6). The acquired equipment was an automatic DNA sequencer ABI 377 from Applied Biosystems. Since the beginning of 2002, counting on the precious technical support of the biologist Sabrina Souza, trained to manage the laboratory with the supervision of responsible professors, the Unit adds up more than 17,500 sequences for professors and researchers of Biochemistry, Biophysics and Pharmacology Departments. Who join the INFAR building and also other departments of UNIFESP, showing that this work became essential throughout these years to the conduction and consolidation of research projects that evolve genomic approaches or gene expression.Even though the success of this enterprise and the necessity of a continuous encourage of DNA sequencing to the develop many projects from the Multi-user Unit Associated Researchers the present proposal has as aim to bring up to date the Automatic DNA sequencing Equipment of this unit, even though the ABI377 model is now deactivated for the lack of reposition pieces due to the discontinue politics of this equipment by Applied Biosystems company (*see attached document to the process carrying the emitted report of Applied Biosystems). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOARES, TATIANE SANCHES; SOARES TORQUATO, RICARDO JOSE; ALVES LEMOS, FRANCISCO JOSE; TANAKA, APARECIDA SADAE. Selective inhibitors of digestive enzymes from Aedes aegypti larvae identified by phage display. Insect Biochemistry and Molecular Biology, v. 43, n. 1, p. 9-16, JAN 2013. (09/05405-3, 05/03514-9)

SOARES, TATIANE SANCHES; SOARES TORQUATO, RICARDO JOSE; GONZALEZ, YAMILE GONZALEZ; ALVES LEMOS, FRANCISCO JOSE; TANAKA, APARECIDA SADAE. Production of serine protease inhibitors by mutagenesis and their effects on the mortality of Aedes aegypti L. larvae. PARASITES & VECTORS, v. 8, OCT 6 2015. (09/05405-3, 09/06003-6, 05/03514-9, 12/03657-8)

SOARES, TATIANE SANCHES; OKUTA WATANABE, RENATA MIDORI; TANAKA-AZEVEDO, ANITA MITICO; SOARES TORQUATO, RICARDO JOSE; LU, STEPHEN; FIGUEIREDO, ANA CARVALHO; BARBOSA PEREIRA, PEDRO JOSE; TANAKA, APARECIDA S.. Expression and functional characterization of boophilin, a thrombin inhibitor from Rhipicephalus (Boophilus) microplus midgut. Veterinary Parasitology, v. 187, n. 3-4, p. 521-528, JUL 6 2012. (09/05405-3, 05/03514-9)