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In vivo targeting of dendritic cells with Hsp60 peptide to induce tolerance of allogeneic murine

Grant number: 10/01161-0
Support type:Regular Research Grants
Duration: May 01, 2010 - October 31, 2012
Field of knowledge:Biological Sciences - Immunology - Cellular Immunology
Principal Investigator:Verônica Porto Carreiro de Vasconcellos Coelho
Grantee:Verônica Porto Carreiro de Vasconcellos Coelho
Home Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Protocols for induction of immune tolerance has been the subject of research of many immunologists seeking to prolong the survival of allogeneic grafts. In this project we have to evaluate the tolerogenic potential of heat shock protein (Hsp60) and dendritic cells in the context of murine allograft. This effect will be evaluated using the strategy of targeting in vivo by administration of anti-DEC-205 coupled to the peptides of Hsp60, N3 and N7, and as the injection of immature dendritic cells (iDC)of donor in interleukin-10 in the presence of peptides N3 and N7. These effects will be evaluated for histopathological analysis and immunohistochemistry of grafts, where appropriate, analysis of markers of regulatory T cells and a panel of molecules associated with immunoregulation in transplanted animals. (AU)