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Disease resistance in citrus: genetic mapping and functional genomics of new hybrids


Although the citriculture is one of the most important economic activities in Brazil, it is based on a small number of varieties. This fact has contributed for the vulnerability of the culture regarding the phytosanitary problems. A higher number of varieties/genotypes with potential for commercial growing, either for the industry or fresh market, has been one of the main objectives of citrus breeding programs.The genetic breeding of citrus has improved, in the last decades, due to the possibility of an association between biotechnological tools and classical methods of breeding. The use of molecular markers for early selection of zygotic seedlings from controlled crosses resulted in the possibility of selection of a high number of new combination and, as a consequence, the establishment of a great number of hybrids in field experiments. The Centro APTA Citros Sylvio Moreira / IAC has launched, since 1997, a wide program of genetic breeding of citrus through controlled crosses. Plant populations obtained from hybridization between several varieties, selected by molecular markers, were recently established in several regions of the São Paulo State resulting in an experimental network for new genotypes. Moreover, other project regarding ESTs sequencing has provided more than 280,000 sequences from different genotypes and challenging conditions. The existence of linkage maps and QTLs already mapped, the development of EST library, altogether make very demanding and urgent the exploration of such data to launch a wider genetic study of citrus. Accordingly, besides the field experiments for phenotypic evaluation, experiments with differential genes expression will be conducted. The validation of genes differentially expressed and their correlation with phenotypic traits will permit the understanding of disease resistance mechanisms and increase the accuracy during hybrids selection.The present proposal aims to continue the citrus breeding program by focusing disease resistance. The proposal has as main focuses: 1) genetic mapping of qualitative and quantitative (QTL) traits and 2) correlation of the phenotypic characteristics and differential gene expression evaluated by microarrays and real time RTqPCR . (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MAURICIO, F. N.; SORATTO, T. A. T.; DIOGO, J. A.; BOSCARIOL-CAMARGO, R. L.; DE SOUZA, A. A.; COLETTA-FILHO, H. D.; SILVA, J. A. A.; MEDEIROS, A. H.; MACHADO, M. A.; CRISTOFANI-YALY, M. Analysis of Defense-Related Gene Expression in Citrus Hybrids Infected by Xylella fastidiosa. PHYTOPATHOLOGY, v. 109, n. 2, p. 301-306, FEB 2019. Web of Science Citations: 1.
SAGAWA, C. H. D.; CRISTOFANI-YALY, M.; NOVELLI, V. M.; BASTIANEL, M.; MACHADO, M. A. Assessing genetic diversity of Citrus by DArT_seq (TM) genotyping. PLANT BIOSYSTEMS, v. 152, n. 4, p. 593-598, 2018. Web of Science Citations: 1.
BOAVA, LEONARDO PIRES; CRISTOFANI-YALY, MARIANGELA; MACHADO, MARCOS ANTONIO. Physiologic, Anatomic, and Gene Expression Changes in Citrus sunki, Poncirus trifoliata, and Their Hybrids After `Candidatus Liberibacter asiaticus' Infection. PHYTOPATHOLOGY, v. 107, n. 5, p. 590-599, MAY 2017. Web of Science Citations: 3.
GOIS, I. B.; BOREM, A.; CRISTOFANI-YALY, M.; DE RESENDE, M. D. V.; AZEVEDO, C. F.; BASTIANEL, M.; NOVELLI, V. M.; MACHADO, M. A. Genome wide selection in Citrus breeding. Genetics and Molecular Research, v. 15, n. 4 2016. Web of Science Citations: 4.
BOAVA, LEONARDO PIRES; DUARTE SAGAWA, CINTIA HELENA; CRISTOFANI-YALY, MARIANGELA; MACHADO, MARCOS ANTONIO. Incidence of `Candidatus Liberibacter asiaticus'-Infected Plants Among Citrandarins as Rootstock and Scion Under Field Conditions. PHYTOPATHOLOGY, v. 105, n. 4, p. 518-524, APR 2015. Web of Science Citations: 7.
BOAVA, LEONARDO P.; CRISTOFANI-YALY, MARINGELA; MAFRA, VALERIA S.; KUBO, KAREN; KISHI, LUCIANO T.; TAKITA, MARCO A.; RIBEIRO-ALVES, MARCELO; MACHADO, MARCOS A. Global gene expression of Poncirus trifoliata, Citrus sunki and their hybrids under infection of Phytophthora parasitica. BMC Genomics, v. 12, JAN 17 2011. Web of Science Citations: 30.

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