Experimental study in rabbits of treatment of model of rhinosinusitis with luffa operculata in water spray

• Grant number: 07/05153-9
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2008
• End date: April 30, 2009
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Luc Louis Maurice Weckx
• Grantee: Luc Louis Maurice Weckx
• Host Institution: Departamento de Otorrinolaringologia. Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The prevalence of infecto-inflammatory diseases of the airways is extremely high in the general population. The treatment of the rhinosinusitis can be done in many ways. Besides, there are a variety of clinical patterns and predisposing factors, as well as associated situations that hinder the understanding of the pathology. The treatment usually indicated to these situations is oral antibiotics for community infectious agents associated to anti-inflammatories. However, the use of topical substances that help the drainage could be useful to these patients. The luffa operculata has been traditionally used as a treatment for sinusitis by the Brazilians. There is no study evaluating its mucinating capacity in animal models throught spray instillation. Objective: to evaluate the effectiveness of the use of the luffa operculata under water spray form in experimental model of bacterial rhinosinusitis. Material and Method: 60 rabbits will be accompanied up to 45 days. The animals will be submitted to proceedings need to achieve an experimental model of bacterial rhinosinusitis in agreement with technique established previously by the authors that consists of blockade of the nasal cavity with sponge associated with the instillation of toxoid of streptococcus and staphylococcus and blood in the maxillary antrum. Once reached the model, a group will be treated with the drug association and other group will receive the vehicle without drug. The animals will be followed for periods of 5 days, 15 days and 30 days. The antri maxillaries will be appraised histological and with culture of its secretion and the results compared with the control and among the intervention groups. All the animals will have samples of blood collected in the 1st. day, 5th. day and day of the euthanasia, for blood count making. The mucous will be collected through time to study its rheological properties. (AU)