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Weeds and host of Meloidogyne javanica M. incognita in cane sugar culture

Grant number: 14/06639-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2014
Effective date (End): June 30, 2015
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:Patrícia Andrea Monquero
Grantee:João Paulo Ruvieri San Gregorio
Home Institution: Centro de Ciências Agrárias (CCA). Universidade Federal de São Carlos (UFSCAR). Araras , SP, Brazil

Abstract

The efficacy in controlling nematodes depends on the knowledge of their host range, to minimize the incidence of these plant parasitic in cane sugar. Because of this, the objective of this experiment is to survey the weeds considered important in cane sugar culture by determining whether these species serve as hosts of the nematode Meloidogyne javanica and M.incognita. The experimental design is a completely randomized, factorial 10 (weeds) x 2 (species of nematodes), with six replicates. The weeds Ipomoea grandifolia (Dammer), Ipomoea purpurea (L.) Roth (Convolvulaceae) Merremia aegypitia (L.) urban (Convolvulaceae), Luffa aegyptiaca Mill. (Cucurbitaceae), Momordica charantia L. (Cucurbitaceae), Ricinus communis L. (Euphorbiaceae), Macroptilium atropurpureum (DC) Urb (Fabaceae), Mucuna aterrima (Piper & Tracy) Holland (Fabaceae), Chloris polydactyla (L.) Sw. (Poaceae) and Digitaria horizontalis Willd. and Urochloa decumbens (Poaceae) will be tested. Each weed species will be sown separately in pots with a capacity of 10L, which will be kept in a greenhouse, and when the weeds are between the second and fourth pair of true leaves (eudicotyledonous) or early tillering (Monocotiledôdneas) will be inoculated with eggs and second stage juveniles (J2) of Meloidogyne javanica and M. incognita. The evaluation will be done at 60 days after inoculation and consist of the processing of the root system of each plant for the extraction of nematodes and after this procedure, the quantification of nematodes will be held in an optical microscope (magnification 50X) with the aid of the camera Petri calculated and the factor of reproduction (FR) of the parasite in each treatment. (AU)