Scholarship 11/19764-5 - Controle biológico, Bactérias - BV FAPESP
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Evaluation of rhizobacteria as agents for biological control of soybean crop nematodes Meloidogyne javanica, M. incognita, Pratylenchus brachyurus, Heterodera glycines and Rotylenchulus reniformis in vitro and in pots

Grant number: 11/19764-5
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Start date until: December 01, 2011
End date until: November 30, 2012
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal Investigator:Pedro Luiz Martins Soares
Grantee:Rafael Bernal de Carvalho
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The plant parasitic nematodes are responsible for important annual losses in world agricultural production. Among the various methods used to control these pathogens the nematodes biological control is an ecological and viable option to traditional methods of control. Among the biological control agents, rhizobacteria are considered promising by many authors. Therefore this study aims to evaluate the potential for biological control of 10 rhizobacteria isolates from the Laboratório de Nematologia do Departamento de Fitossanidade da UNESP / FCAV, Campus de Jaboticabal, to the important soybean nematodes in Brazil: Meloidogyne javanica M. incognita, Pratylenchus brachyurus, Rotylenchulus reniformis and Heterodera glycines in vitro by evaluating mortality and ovicidal action of nematode active forms for bacterial suspensions adjusted to an optical density (OD540) of 0.5 in a completely randomized design with four replications, and the results subsequently subjected to analysis of variance by F test, if it's significant, the treatment means will be compared by the Tukey test at 5% probability. After the selection of promising isolates they will be used for seed treatment (ST) of a soybean cultivar host species of nematodes which will also be treated with inoculant Bradyrrhizbium japonicum-based, distributed in a completely randomized design with five replicates, each replicate consists of a pot. Will be evaluated later germination percentage, plant height, shoot fresh weight, root fresh weight, number of pods, number of nodes, number of galls and number of nematodes in the roots, and number of nematodes per 100 cm3 of soil. These variables are then subjected to analysis of variance by F test, if significant, the treatment means will be compared by Tukey test at 5% probability. (AU)

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