Development of oral microbiota, prevalence and transmissibility of Streptococcus salivarius in infants from 0 to 6 months

• Grant number: 09/08355-7
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2009
• End date: August 31, 2010
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Claudia Ota-Tsuzuki
• Grantee: Claudia Ota-Tsuzuki
• Host Institution: Universidade de Guarulhos (UNG). Campus Guarulhos-Centro. Guarulhos , SP, Brazil

Abstract

Although the absence of microorganisms in the oral cavity of infants at birth, in the course of the first months of life, the physiological changes and the exposure to the environmental microorganisms might influence the profile of oral microbiota. Since S. salivarius is the one of the most numerous and one of the first colonizers found in the oral cavity of infants, this study aims to investigate the transmissibility and a possible activity of salivaricin against other members of oral microbiota during the first months of life. For this purpose: 30 female volunteers and their infants will have saliva and oral mucosa and subgingival biofilm been collected. The biofilm and the bacterial isolates will be analyzed: 1) by checkerboard DNA-DNA hybridization technique, the microbiological profile of the mothers and infants will be accessed; 2) clinical isolates from mothers and infants will be genotyped by AP-PCR in order to verify the transmissibility and maintenance of this species during the first months of life; and 3) determine the prevalence of S. salivarius and, among the clinical isolates, determine the salivaricin producing and not producing strains during the first 6 months age (AU)