| Grant number: | 07/08405-9 |
| Support Opportunities: | Regular Research Grants |
| Start date: | April 01, 2008 |
| End date: | March 31, 2010 |
| Field of knowledge: | Biological Sciences - Physiology - Physiology of Organs and Systems |
| Principal Investigator: | Maria Esméria Corezola do Amaral |
| Grantee: | Maria Esméria Corezola do Amaral |
| Host Institution: | Pró-Reitoria de Pós-Graduação e Pesquisa. Centro Universitário Hermínio Ometto (UNIARARAS). Fundação Hermínio Ometto (FHO). Araras , SP, Brazil |
Abstract
In recent decades, the scientific community has shown great interest in studies on the signaling pathways involving protein regulators of the disease's development such as obesity and diabetes. For 2025, it is estimated that the prevalence of diabetes mellitus is around 9% of the adult population. Obesity and diabetes mellitus are multifactor syndromes and recent studies indicate that the development of them is closely related to imbalance on food intake. Beyond the imbalance on food intake, modulate environmental and genetic factors induce the onset of these diseases. In this context, the emphasis given to the control of food intake, specifically for caloric restriction, emerges as the modulator of metabolic pathways preventing such diseases. Caloric restriction active cellular mechanisms responsible delayed of the onset of such syndromes, promoting survival of these individuals. These benefits, especially in subject longevity are assigned to regulatory proteins that on the one hand reduce the secretion of insulin and, secondly increase peripheral sensitivity to this hormone. Among the proteins that are activated or not, depending on caloric restriction and coordinate the secretion and signaling pathway of insulin, the protein deserves emphasis of the family Sirtuins and AMPK. Sirt 1 and Sirt 4 are highly expressed in the pancreatic islets and have a fine control on the secretion of insulin. The Sirt 1 behaves as the control positive and Sirt 4 as repressor of the insulin secretion. In addition, the AMPK is part of the signaling pathway of metformin and participates in many metabolic pathways. The AMPK inhibits gluconeogenesis, the fatty acids and cholesterol synthesis. So when AMPK is active it control weight and increased peripheral sensitivity to insulin. Thus, the goal of this study is to evaluate the effect of caloric restriction of 60% with respect to control over gene expression and protein of Sirt 1 and Sirt 4 in secretion of insulin both in vivo and from isolated pancreatic islets. Also, determine the expression of AMPK, depending on caloric restriction on insulin secretion from both in vivo and in vitro. This project can provide new strategies to prevent diseases such as diabetes and obesity promoting longevity. (AU)
| Articles published in Agência FAPESP Newsletter about the research grant: |
| More itemsLess items |
| TITULO |
| Articles published in other media outlets ( ): |
| More itemsLess items |
| VEICULO: TITULO (DATA) |
| VEICULO: TITULO (DATA) |