Effect of vitamin E on insulin action and secretion in animals subjected to caloric restriction

• Grant number: 12/01146-6
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2012 - June 30, 2014
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Maria Esméria Corezola do Amaral
• Grantee: Maria Esméria Corezola do Amaral
• Host Institution: Pró-Reitoria de Pós-Graduação e Pesquisa. Centro Universitário Hermínio Ometto (UNIARARAS). Fundação Hermínio Ometto (FHO). Araras , SP, Brazil

Abstract

Type 2 diabetes is a chronic disease, a global health problem. It is a disease characterized by absolute or relative deficiency of the action / insulin secretion associated with disturbances in the metabolism of carbohydrates, lipids and proteins. This is because their relationship with aging that affects the body's energy metabolism. Many studies suggest that oxidative stress in cells may be responsible for the development of metabolic disorders presented by the diabetic. The use of antioxidants along with a proper diet seems to be a therapeutic strategy for the individual to delay the onset of pre-diabetes, diabetes and accordingly save the functional exhaustion of the pancreas. Few studies on vitamin E versus calorie restriction (CR) in the literature are not well established and the benefits of this vitamin in action and insulin secretion. Already, the RC is studied as an effective way to increase life expectancy in many species, being responsible for a healthy and a strong regime against metabolic diseases. The classic mechanism that could explain the effect of reducing the calorie intake is related to reduced body fat, insulin, reactive oxygen species produced during respiration that cause oxidative damage to DNA and RNA of cells. To establish the effect of micronutrient supplementation and antioxidant, alpha-tocopherol (vitamin E), with the CR in insulin action and secretion, this project aims at the impact of diet on biological mechanisms for the prevention of health. For this purpose will be assessed for insulin secretion in islets isolated by different stimuli, tests that indicate the improvement in insulin sensitivity like IR, AKT and GLUT4, analysis of the beta cell mass, lipid profile and study of proteins involved in insulin action and the benefits of CR, as SIRT1, SIRT4 and muscarinic receptors, M1 and M3. Therefore, the study of vitamin E by the intervention of the CR becomes interesting point of view that goes beyond the conventional treatment against diabetes and associated diseases. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOLIM, LEONARDO VINICIUS; COREZOLA DO AMARAL, MARIA ESMERIA; FACHI, JOSE LUIS; MENDES, MAIRA FELONATO; DE OLIVEIRA, CAMILA ANDREA. Vitamin E and caloric restriction promote hepatic homeostasis through expression of connexin 26, N-cad, E-cad and cholesterol metabolism genes. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 39, p. 86-92, JAN 2017. (12/01146-6)

VENTURINI, PAULA R.; THOMAZINI, BRUNA FONTANA; OLIVEIRA, CAMILA ANDREA; ALVES, ARMINDO A.; CAMARGO, THAIS FURTADO; DOMINGUES, CAIO E. C.; BARBOSA-SAMPAIO, HELENA C. L.; DO AMARAL, MARIA ESMERIA C.. Vitamin E supplementation and caloric restriction promotes regulation of insulin secretion and glycemic homeostasis by different mechanisms in rats. BIOCHEMISTRY AND CELL BIOLOGY, v. 96, n. 6, p. 777-785, DEC 2018. (12/01146-6)