Characterization of cadmium transport and its interaction with calcium on epithelial cells of Ucides cordatus, a mangrove crab

• Grant number: 09/15546-3
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2010
• End date: October 31, 2013
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Flavia Pinheiro Zanotto
• Grantee: Flavia Pinheiro Zanotto
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Crustaceans belong to a diverse group of animals, and are found in estuarine, marine, freshwater and terrestrial habitats. Functional alterations during the evolution of this group have led to specialized adaptations of epithelial cells found in gills, hypodermis, hepatopancreas and antennal gland (analogous to the kidneys), where they regulate the transport of molecules and ions between the environment and the hemolymph. Toxic metals can accumulate in the animals through hypodermis and gills, or through the hepatopancreas. This project will study the transport of a metal, cadmium (Cd) and its interaction with calcium (Ca) in isolated cells of hepatopancreas and gills of Ucides cordatus, a mangrove crab, inhabitant of regions vulnerable to metal pollution. The methodology will involve the use of fluorescent markers for Cd (FluoZin 1) and oxidative stress biomarkers, to quantify the deleterious effects of this metal. In this way, this study could be used as a theoretical support for the effects of a metal Cd2+ in interaction with Ca2+, an essential ion for crustaceans, both at the whole animal and cellular level. Moreover, the study of a metal and its transport could help the understanding of the biological potential of crabs as biomarkers of ambient pollution. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEITE, V. P.; ZANOTTO, F. P.. Calcium transport in gill cells of Ucides cordatus, a mangrove crab living in variable salinity environments. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 166, n. 2, p. 370-374, OCT 2013. (09/15546-3)

ORTEGA, PRISCILA; VITORINO, HECTOR A.; MOREIRA, RENATA G.; PINHEIRO, MARCELO A. A.; ALMEIDA, ALAOR A.; CUSTODIO, MARCIO R.; ZANOTTO, FLAVIA P.. Physiological differences in the crab Ucides cordatus from two populations inhabiting mangroves with different levels of cadmium contamination. Environmental Toxicology and Chemistry, v. 36, n. 2, p. 361-371, FEB 2017. (09/15546-3)

ORTEGA, P.; CUSTODIO, M. R.; ZANOTTO, F. P.. Characterization of cadmium plasma membrane transport in gills of a mangrove crab Ucides cordatus. AQUATIC TOXICOLOGY, v. 157, p. 21-29, DEC 2014. (09/15546-3)

SA, M. G.; ZANOTTO, F. P.. Characterization of copper transport in gill cells of a mangrove crab Ucides cordatus. AQUATIC TOXICOLOGY, v. 144, p. 275-283, NOV 15 2013. (09/15546-3)

ORTEGA, PRISCILA; SANTOS, RUDNEY A.; LACOUTH, PATRICIA; ROZAS, ENRIQUE E.; CUSTODIO, MARCIO R.; ZANOTTO, FLAVIA PINHEIRO. Caracterização citoquímica de células de brânquias e hepatopâncreas de Ucides cordatus (Crustacea, Brachyura) validado por transporte celular de metais. IHERINGIA SERIE ZOOLOGIA, v. 104, n. 3, p. 347-354, SEP 30 2014. (09/15546-3)

ORTEGA, PRISCILA; CUSTODIO, MARCIO R.; ZANOTTO, FLAVIA P.. Characterization of cadmium transport in hepatopancreatic cells of a mangrove crab Ucides cordatus: The role of calcium. AQUATIC TOXICOLOGY, v. 188, p. 92-99, JUL 2017. (09/15546-3)