Evaluation of immune response in mice immunized with Semliki Forest Virus (SFV) carrying the genetic information for in vivo synthesis of rabies virus glycoprotein (RVGP)

• Grant number: 10/08742-8
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2010
• End date: September 30, 2012
• Field of knowledge: Biological Sciences - Immunology
• Principal Investigator: Renato Mancini Astray
• Grantee: Renato Mancini Astray
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Carlos Augusto Pereira

Abstract

The Semliki Forest Virus (SFV) expression system has been utilized for in vitro expression of several molecules of biotechnology interest, and for in vivo expression of medical interest molecules. Known by the ability to infect several cell types, mainly mammalian cells, the SFV has been proposed as a reliable vaccine vector. However, little information is available about its immunologic and adjuvant properties and its utilization is limited for some technologic constraints, as the imprecise viral titration methodology. In this project, infectious but non-replicative recombinant SFV particles will be obtained from plasmids by molecular biology techniques. These particles will bear the genetic information of the rabies virus glycoprotein (RVGP) and delivery it for in vivo synthesis in mice cells. These particles will be able to induce neutralizing antibodies in immunized mice, as already evaluated in preliminary assays. In order to better determination of virus titers, it will be established a new qRT-PCR methodology, based in the quantification of viral RNA in viral lots. The titrated particles will be utilized for mice immunization and the pattern of immune response will be determined, analyzing humoral and cellular responses. Immunized mice will be eventually challenged against rabies virus infection, in order to determine the potency of SFV-RVGP vaccination. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SUAREZ-PATINO, SANDRA FERNANDA; MANCINI, RENATO ASTRAY; PEREIRA, CARLOS AUGUSTO; TORRES SUAZO, CLAUDIO ALBERTO; MENDONCA, RONALDO ZUCATELLI; CALIL JORGE, SORAIA ATTIE. Transient expression of rabies virus glycoprotein (RVGP) in Drosophila melanogaster Schneider 2 (S2) cells. Journal of Biotechnology, v. 192, n. A, p. 255-262, DEC 20 2014. (10/08742-8, 11/08331-0, 02/09482-3)

PUGLIA, ANA L. P.; REZENDE, ALEXANDRE G.; JORGE, SORAIA A. C.; WAGNER, RENAUD; PEREIRA, CARLOS A.; ASTRAY, RENATO M.. Quantitative RT-PCR for titration of replication-defective recombinant Semliki Forest virus. Journal of Virological Methods, v. 193, n. 2, p. 647-652, NOV 2013. (11/15269-0, 10/08742-8)

SUAREZ-PATINO, SANDRA FERNANDA; MANCINI, RENATO ASTRAY; PEREIRA, CARLOS AUGUSTO; TORRES SUAZO, CLAUDIO ALBERTO; MENDONCA, RONALDO ZUCATELLI; CALIL JORGE, SORAIA ATTIE. Transient expression of rabies virus glycoprotein (RVGP) in Drosophila melanogaster Schneider 2 (S2) cells. Journal of Biotechnology, v. 192, p. 8-pg., 2014-12-20. (11/08331-0, 10/08742-8, 02/09482-3)