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Study of repetitive elements present in the genome of species of Anostomidae (Pisces, Characiformes)

Abstract

While most fish species do not show sex chromosomes differentiated, there is a great diversity concerning the mechanisms of chromosomal sex determination, including heterogametic females or males and single or multiple systems. In most cases, the difference between sex chromosomes involve heterochromatic blocks and this fact is well established in ZZ:ZW system, where loss or gain of heterochromatin are the main causes of differentiation between these chromosomes. The chromosomes of Leporinus (ZZ / ZW) have been studied in our laboratory, with interesting results, especially with respect to repetitive sequences located on sex chromosomes. Despite the new information, little is known about the evolution of sex chromosomes and sex determination in fish. Thus, this project aims to mapping repetitive sequences and total chromosomes already obtained by enzymatic digestion, chromosome microdissection and flow sorting. Also other possible markers sequences and chromosomes will be isolate. All the probes will be used in different Anostomidae species and comparisons among species and among different populations will be done in order to find markers for species/population specific and to understand the karyotype evolution of the group studied. The results may provide valuable information for understanding the structural and functional organization and evolution of the fish genome. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PONZIO, JULIA CHAGAS; PISCOR, DIOVANI; PARISE-MALTEMPI, PATRICIA PASQUALI. Chromosomal locations of U2 snDNA clusters in Megaleporinus, Leporinus and Schizodon (Characiformes: Anostomidae). BIOLOGIA, v. 73, n. 3, p. 295-298, MAR 2018. Web of Science Citations: 0.

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