Chemical and structural analysis of Xylella fastidiosa biofilms

• Grant number: 10/51748-7
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2010
• End date: March 31, 2013
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Mônica Alonso Cotta
• Grantee: Mônica Alonso Cotta
• Host Institution: Instituto de Física Gleb Wataghin (IFGW). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

In this project, we plan to incorporate functionalization routines for surfaces and tips of atomic force microscopy to study mechanical and chemical properties of biomolecules (proteins) and the membrane of the plant pathogen Xylella fastidiosa. Several microscopy techniques (atomic force and force spectroscopy, optical and fluorescence, confocal) will be used. With this proposal we expect to gather information about the structural properties of these biomolecules and their role in the formation of biofllms that cause vascular occlusion of the xylem of affected plants. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VAZ, JULIANA M.; TAKETA, THIAGO B.; HERNANDEZ-MONTELONGO, JACOBO; CHEVALLIER, PASCALE; COTTA, MONICA A.; MANTOVANI, DIEGO; BEPPU, MARISA M.. Antibacterial properties of chitosan-based coatings are affected by spacer-length and molecular weight. Applied Surface Science, v. 445, p. 478-487, JUL 1 2018. (13/14888-3, 10/51748-7, 13/10957-0, 13/05135-1)

HERNANDEZ-MONTELONGO, JACOBO; NASCIMENTO, VICENTE F.; MURILLO, DUBER; TAKETA, THIAGO B.; SAHOO, PRASANA; DE SOUZA, ALESSANDRA A.; BEPPU, MARISA M.; COTTA, MONICA A.. Nanofilms of hyaluronan/chitosan assembled layer-by-layer: An antibacterial surface for Xylella fastidiosa. Carbohydrate Polymers, v. 136, p. 1-11, JAN 20 2016. (13/02300-1, 10/51748-7)

SAHOO, PRASANA K.; JANISSEN, RICHARD; MONTEIRO, MONIELLEN P.; CAVALLI, ALESSANDRO; MURILLO, DUBER M.; MERFA, MARCUS V.; CESAR, CARLOS L.; CARVALHO, HERNANDES F.; DE SOUZA, ALESSANDRA A.; BAKKERS, ERIK P. A. M.; et al. Nanowire Arrays as Cell Force Sensors To Investigate Adhesin-Enhanced Holdfast of Single Cell Bacteria and Biofilm Stability. Nano Letters, v. 16, n. 7, p. 4656-4664, JUL 2016. (13/02300-1, 10/51748-7)

HERNANDEZ-MONTELONGO, J.; LUCCHESI, E. G.; GONZALEZ, I.; MACEDO, W. A. A.; NASCIMENTO, V. F.; MORAES, A. M.; BEPPU, M. M.; COTTA, M. A.. Hyaluronan/chitosan nanofilms assembled layer-by-layer and their antibacterial effect: A study using Staphylococcus aureus and Pseudomonas aeruginosa. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 141, p. 499-506, MAY 1 2016. (13/02300-1, 10/51748-7)

DA SILVA, ALDELIANE M.; SAHOO, PRASANA K.; CAVALLI, ALESSANDRO; DE SOUZA, ALESSANDRA A.; BAKKERS, ERIK P. A. M.; CESAR, CARLOS L.; JANISSEN, RICHARD; COTTA, MONICA A.. Nanowire Arrays as Force Sensors with Super-Resolved Localization Position Detection: Application to Optical Measurement of Bacterial Adhesion Forces. SMALL METHODS, v. 2, n. 7, JUL 10 2018. (13/02300-1, 10/51748-7, 13/10957-0)

HERNANDEZ-MONTELONGO, J.; LUCCHESI, E. G.; NASCIMENTO, V. F.; FRANCA, C. G.; GONZALEZ, I.; MACEDO, W. A. A.; MACHADO, D.; LANCELLOTTI, M.; MORAES, A. M.; BEPPU, M. M.; et al. Antibacterial and non-cytotoxic ultra-thin polyethylenimine film. Materials Science & Engineering C-Materials for Biological Applications, v. 71, p. 718-724, FEB 1 2017. (13/02300-1, 10/51748-7)

JANISSEN, RICHARD; MURILLO, DUBER M.; NIZA, BARBARA; SAHOO, PRASANA K.; NOBREGA, MARCELO M.; CESAR, CARLOS L.; TEMPERINI, MARCIA L. A.; CARVALHO, HERNANDES F.; DE SOUZA, ALESSANDRA A.; COTTA, MONICA A.. Spatiotemporal distribution of different extracellular polymeric substances and filamentation mediate Xylella fastidiosa adhesion and biofilm formation. SCIENTIFIC REPORTS, v. 5, APR 20 2015. (10/18107-8, 10/51748-7)

MONTEIRO, MONIELLEN P.; HERNANDEZ-MONTELONGO, JACOBO; SAHOO, PRASANA K.; MONTELONGO, ROSAURA HERNANDEZ; DE OLIVEIRA, DOUGLAS S.; PIAZZETA, MARIA H. O.; GARCIA SANDOVAL, JUAN P.; DE SOUZA, ALESSANDRA A.; GOBBI, ANGELO L.; COTTA, MONICA A.. Functionalized microchannels as xylem-mimicking environment: Quantifying X. fastidiosa cell adhesion. BIOPHYSICAL JOURNAL, v. 120, n. 8, p. 1443-1453, APR 20 2021. (10/51748-7, 15/16611-4, 13/10957-0)

JANISSEN, RICHARD; SAHOO, PRASANA K.; SANTOS, CLELTON A.; DA SILVA, ALDELIANE M.; VON ZUBEN, ANTONIO A. G.; SOUTO, DENIO E. P.; COSTA, ALEXANDRE D. T.; CELEDON, PAOLA; ZANCHIN, NILSON I. T.; ALMEIDA, DIOGO B.; et al. InP Nanowire Biosensor with Tailored Biofunctionalization: Ultrasensitive and Highly Selective Disease Biomarker Detection. Nano Letters, v. 17, n. 10, p. 5938-5949, OCT 2017. (13/02300-1, 10/51748-7, 15/16611-4)

LORITE, GABRIELA S.; JANISSEN, RICHARD; CLERICI, JOAO H.; RODRIGUES, CAROLINA M.; TOMAZ, JUAREZ P.; MIZAIKOFF, BORIS; KRANZ, CHRISTINE; DE SOUZA, ALESSANDRA A.; COTTA, MONICA A.. Surface Physicochemical Properties at the Micro and Nano Length Scales: Role on Bacterial Adhesion and Xylella fastidiosa Biofilm Development. PLoS One, v. 8, n. 9, SEP 20 2013. (06/06397-6, 10/51748-7)

LEHNER, BENJAMIN A. E.; BENZ, DOMINIK; MOSHKALEV, STANISLAV A.; MEYER, ANNE S.; COTTA, MONICA A.; JANISSEN, RICHARD. Biocompatible Graphene Oxide Nanosheets Densely Functionalized with Biologically Active Molecules for Biosensing Applications. ACS APPLIED NANO MATERIALS, v. 4, n. 8, p. 8334-8342, AUG 27 2021. (10/51748-7, 10/18107-8, 13/02300-1, 15/16611-4, 12/51580-4)