Development of FIA systems involving pervaporation and enzymatic immobilization for determination of chemical groups of agroindustrial interest

• Grant number: 97/03305-2
• Support Opportunities: Regular Research Grants
• Start date: August 01, 1997
• End date: July 31, 1999
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Elias Ayres Guidetti Zagatto
• Grantee: Elias Ayres Guidetti Zagatto
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Analytical methods will be developed based on systems of chemical analysis by flow injection and non-chromatographic continuous separation for the monitoring of ethanol in an ethanolic fermentation, as well as the determination of reductive sugars; in addition, the determination of urea in samples of agronomic interest, especially, soils and fertilizers will be undertaken. The determination of ethanol will be carried out via monitoring of hydrogen peroxide formed through the enzymatic reaction; the reductive sugars will be determined by means of ion-selective electrodes with sensor immobilized in PVC. The determination of urea will be carried out by means of enzymatic reaction (immobilized urease). (AU)