Candidate gene identification for angular leaf spot in common bean (Phaseolus vulgaris L.)

• Grant number: 10/51673-7
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2010
• End date: August 31, 2013
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Luciana Lasry Benchimol-Reis
• Grantee: Luciana Lasry Benchimol-Reis
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Campinas , SP, Brazil
• City of the host institution: Campinas

Abstract

Common bean (Phaseolus vulgaris L.) is a legume of great importance in human nutrition. Common bean breeding search tools that improve transfer of resistance genes to developing cultivars. Genomic tools permit to label, introgress and clone genes or QTLs through molecular markers. Resistance gene mapping enable gene, epistatic and environmental interactions studies among these QTLs, providing important information to breeding programs. The angular leaf spot, caused by the fungus Pseudocercospora griseola (Sacc.) Crous & U. Braun, causes large losses to common bean producers. Thus, genetically resistant cultivars are desirable. Fine mapping allows identification of specific allele molecular markers that co-segregate with resistance loci, making positional cloning possible. Therefore, breeder can manipulate the genetic variation, introgressing and pyramiding resistance genes through marker assisted selection or by transgenic procedures. Taking into account the continuing of the FAPESP process n° 05/53819-0 (Mapping of anthracnose and angular leaf spot resistance loci in common bean using microsatellite markers) and the FAPESP scholarship process n° 06/58332-5 ["Construction of a molecular-genetic map and mapping loci associated with angular leaf spot resistance in common bean (Phaseolus vulgaris L.) using microsatellite markers"], this project aims mapping angular leaf spot resistance and identify epistatic effects and environment interactions [linked to FAPESP process no 09/02411-2 Doctor's degree scholarship,-"Fine Mapping of loci associated to angular leaf spot resistance in common bean (Phaseolus vulgaris L)"]. The magnitude of the loci identified will be quantified. The region of the major effect locus will be studied in greater detail through its saturation with molecular markers. These markers will be used to identify candidate genes for positional cloning. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DENIS BASSI; BORIS BRIÑEZ; JULIANA SANTA ROSA; PAULA RODRIGUES OBLESSUC; CALÉO PANHOCA DE ALMEIDA; STELLA MARIS NUCCI; LARISSA CHARIEL DOMINGOS DA SILVA; ALISSON FERNANDO CHIORATO; ROSANA PEREIRA VIANELLO; LUIS EDUARDO ARANHA CAMARGO; et al. Linkage and mapping of quantitative trait loci associated with angular leaf spot and powdery mildew resistance in common beans. GENETICS AND MOLECULAR BIOLOGY, v. 40, n. 1, p. 109-122, 2017-02-20. (10/51673-7)

OBLESSUC, PAULA R.; MATIOLLI, CLEVERSON C.; CHIORATO, ALISSON F.; CAMARGO, LUIS E. A.; BENCHIMOL-REIS, LUCIANA L.; MELOTTO, MAELI. Common bean reaction to angular leaf spot comprises transcriptional modulation of genes in the ALS10.1 QTL. FRONTIERS IN PLANT SCIENCE, v. 6, MAR 12 2015. (10/51673-7)

OBLESSUC, PAULA RODRIGUES; BARONI, RENATA MORO; PEREIRA, GUILHERME DA SILVA; CHIORATO, ALISSON FERNANDO; MORAIS CARBONELL, SERGIO AUGUSTO; BRINEZ, BORIS; DA COSTA E SILVA, LUCIANO; FRANCO GARCIA, ANTONIO AUGUSTO; ARANHA CAMARGO, LUIS EDUARDO; KELLY, JAMES D.; et al. Quantitative analysis of race-specific resistance to Colletotrichum lindemuthianum in common bean. MOLECULAR BREEDING, v. 34, n. 3, p. 1313-1329, OCT 2014. (09/02411-2, 10/51673-7)

BASSI, DENIS; BRINEZ, BORIS; ROSA, JULIANA SANTA; OBLESSUC, PAULA RODRIGUES; DE ALMEIDA, CALEO PANHOCA; NUCCI, STELLA MARIS; DOMINGOS DA SILVA, LARISSA CHARIEL; CHIORATO, ALISSON FERNANDO; VIANELLO, ROSANA PEREIRA; ARANHA CAMARGO, LUIS EDUARDO; et al. Linkage and mapping of quantitative trait loci associated with angular leaf spot and powdery mildew resistance in common beans. GENETICS AND MOLECULAR BIOLOGY, v. 40, n. 1, p. 14-pg., 2017-01-01. (10/51673-7)

OBLESSUC, PAULA RODRIGUES; BARONI, RENATA MORO; FRANCO GARCIA, ANTONIO AUGUSTO; CHIORATTO, ALISSON FERNANDO; MORAIS CARBONELL, SERGIO AUGUSTO; ARANHA CAMARGO, LUIS EDUARDO; BENCHIMOL, LUCIANA LASRY. Mapping of angular leaf spot resistance QTL in common bean (Phaseolus vulgaris L.) under different environments. BMC GENETICS, v. 13, JUN 27 2012. (10/51673-7)

OBLESSUC, PAULA RODRIGUES; KUPPER CARDOSO PERSEGUINI, JULIANA MORINI; BARONI, RENATA MORO; CHIORATO, ALISSON FERNANDO; MORAIS CARBONELL, SERGIO AUGUSTO; COSTA MONDEGO, JORGE MAURICIO; VIDAL, RAMON OLIVEIRA; ARANHA CAMARGO, LUIS EDUARDO; BENCHIMOL-REIS, LUCIANA LASRY. Increasing the density of markers around a major QTL controlling resistance to angular leaf spot in common bean. THEORETICAL AND APPLIED GENETICS, v. 126, n. 10, p. 2451-2465, OCT 2013. (09/02411-2, 10/51673-7)