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Population genomics and GWAS for disease resistance and identification of Andean pool introduction regions in carioca bean access

Grant number: 19/19670-2
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): November 01, 2019
Effective date (End): April 30, 2021
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:Luciana Lasry Benchimol-Reis
Grantee:Caléo Panhoca de Almeida
Home Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil


The common bean (Phaseolus vulgaris L.) is a species with primary centers of diversity on the American continent. Due to the continuous process of introduction of new genotypes, some collections contain a high number of accessions, making it difficult to use the existing variability in germplasm banks for conservation and genetic improvement purposes. In addition, it is common to cross elite cultivars to preserve the agronomic characteristics that govern market acceptance, drastically restricting the basis and genetic variability of the improved materials, resulting in losses such as the constant breakdown of resistance to the main diseases of the crop. Among the steps of genetic improvement, the genetic characterization and understanding of the variability present in the main accessions of a breeding program are essential, since it allows the broadening of the base and variability of future cultivars through the selection of more contrasting parents to compose the crosses of the breeding program. In this sense, the present work aims to characterize the structure and genetic diversity of 151 main accessions of common Carioca beans present in the Germplasm Bank of the Agronomic Institute Breeding Program, which were genotyped by BeadChip (BARCBean6K_3) composed of a set of 6,000 SNPs (Proc. FAPESP 2017/24711-4; BEPE FAPESP 2018/15526-1), as well as identify regions of the genome associated with resistance to Pseudocercospora griseola, Colletotrichum lindemuthianum and Fusarium oxysporum f sp. phaseoli through the wide genomic association study (GWAS). The QTNs found for angular spot, anthracnose and fusarium wilt will be validated in a segregating population already genotyped via GBS in a previous project (Proc. FAPESP 2014/11145-2), which enabled the identification of 5,000 high quality SNPs and the construction of a genetic map based on 91 F4RC22 strains, derived from the crossing of two cultivars ['IAC Milênio' x 'AND-277'] and that present contrasting resistance for several diseases. This study is expected to provide evidence on the assesses´ genetic structure such as interrelationships and genetic bases over the years of genetic improvement of carioca beans in Brazil and the application of this information to aid the development of new elite cultivars with desirable traits and resistance to important diseases, allowing the identification of candidate genes of interest to the common bean breeding programs. (AU)