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T cell clonal expansion and proviral load levels among human t cell lymphotropic virus type 1 (HTLV-1) asymptomatic carriers

Grant number: 10/08550-1
Support type:Regular Research Grants
Duration: December 01, 2010 - November 30, 2012
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Ester Cerdeira Sabino
Grantee:Ester Cerdeira Sabino
Home Institution: Hemocentro de São Paulo. Fundação Pró-Sangue. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Assoc. researchers:Sabri Saeed Mohamed Ahmed Al-Sanabani ; Walter Kleine Neto


INTRODUCTION:Early prediction of Adult T Cell Leukemia (ATL) in HTLV-1 carriers can be difficult before a disease becomes clinically evident. In this study, we aimed to determine the rate of T cell clonality among HTLV-1 asymptomatic carriers with different titers of proviral load (PL) and to establish a criterion for follow up for those who will present higher PL.METHODS:A sensitive SYBR Green real time PCR will be applied on mononuclear cells from a group of 261 HTLV-1 asymptomatic subjects followed in our institute since march 1997. PL will be classified into three categories; low titer (50 copies/1000 PBMCs), intermediate titer (50-100 copies/1000 PBMCs) and high titer (above 100 copies/1000 PBMCs). Approximately 213 samples will be assessed for their T cell receptor rearrangements by etablished TCRg multiplex PCR using primers able to amplify all Vg and Jg regions. The size and profile of PCR products will be determined using GeneMapper v 3.2-computer software. Appropriate statistical methods will be used and data of T cell clonality will be cross-referenced to PL to allow for subsequent association studies. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANABANI, SABRI SAEED; NUKUI, YOUKO; PEREIRA, JULIANA; DA COSTA, ANTONIO CHARLYS; SOARES DE OLIVEIRA, ANA CAROLINA; PESSOA, RODRIGO; LEAL, FABIO EUDES; SEGURADO, ALUISIO C.; KALLAS, ESPER GEORGES; SABINO, ESTER CERDEIRA. Lack of evidence to support the association of a single IL28B genotype SNP rs12979860 with the HTLV-1 clinical outcomes and proviral load. BMC INFECTIOUS DISEASES, v. 12, DEC 23 2012. Web of Science Citations: 10.
NETO, WALTER K.; DA-COSTA, ANTONIO C.; DE OLIVEIRA, ANA CAROLINA S.; MARTINEZ, VANESSA P.; NUKUI, YOUKO; SABINO, ESTER C.; SANABANI, SABRI S. Correlation between LTR point mutations and proviral load levels among Human T cell Lymphotropic Virus type 1 (HTLV-1) asymptomatic carriers. VIROLOGY JOURNAL, v. 8, DEC 13 2011. Web of Science Citations: 6.

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