Citric acid root conditioning and biological effects of topically-applied bFGF and BMP-7 on cells relevant for periodontal regeneration

• Grant number: 10/11749-4
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2010
• End date: February 28, 2013
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: José Eduardo Cezar Sampaio
• Grantee: José Eduardo Cezar Sampaio
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Carlos Rossa Junior

Abstract

Significant periodontal regeneration is still unpredictable and root conditioning is one of the first therapeutic approaches developed with this intent. There is great controversy in regard to the conditioning agents and methods of application. Our research group has systematically evaluated the main conditioning agents used (citric acid, tetracycline and EDTA), optimizing conditions for their topical use based on the extent of smear layer removal, exposure of collagen fibrils and adhesion of blood clot and cells. The literature shows promising results for periodontal regeneration with the use of root conditioning in pre-clinical in vivo models, but the results of clinical studies using root conditioning as a sole technique or combined with other techniques are, in general, disappointing. Other possible advantages of chemical root conditioning include: 1) the exposure of endogenous growth factors and biological mediators stored in the dentin or cementum matrix; and 2) the increased adsorption of exogenous growth factors topically applied to the root surfaces. bFGF and BMP-7 are growth factors that present biological effects that may support periodontal regeneration, by increasing angiogenesis and proliferation and cell differentiation and extracellular matrix production, respectively. There is relatively few information on the role of these growth factors in periodontal regeneration, especially on their effects in cells that play a prominent role in periodontal regeneration, namely cementoblasts, periodontal ligament fibroblasts and bone marrow stromal cells. Based on this information, our hypothesis is that root conditioning with citric acid increases adsorption of topically applied growth factors and positively impacts their effects on cells relevant to periodontal regeneration. To test this hypothesis, we propose the following specific aims:-Specific Aim #1: To determine the effect of root conditioning with citric acid on the release profile of topically applied BMP-7 and bFGF-Specific Aim #2: Describe the biological effects of topically-applied bFGF and BMP-7, independently and combined, on proliferation and gene expression profile of cells relevant for periodontal regeneration (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GODOY ROCHA, FERNANDA REGINA; CHAVES DE SOUZA, JOAO ANTONIO; GUIMARAES-STABILI, MORGANA RODRIGUES; CEZAR SAMPAIO, JOSE EDUARDO; ROSSA JUNIOR, CARLOS. Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration. Journal of Applied Oral Science, v. 25, n. 6, p. 689-699, NOV-DEC 2017. (10/11749-4)

GODOY ROCHA, FERNANDA REGINA; CHAVES DE SOUZA, JOAO ANTONIO; GUIMARAES-STABILI, MORGANA RODRIGUES; CEZAR SAMPAIO, JOSE EDUARDO; ROSSA JUNIOR, CARLOS. Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration. Journal of Applied Oral Science, v. 25, n. 6, p. 11-pg., 2017-11-01. (10/11749-4)