Effects of diode laser and fibroblast grow factor on the periodontal regeneration after delayed tooth replantation: radiographic, histological and immunohistochemical analyses in rats

• Grant number: 11/09121-0
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2011 - August 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Denise Pontes Raldi
• Grantee: Denise Pontes Raldi
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade de Taubaté (UNITAU). Taubaté , SP, Brazil
• Associated researchers: Ana Lia Anbinder ; Erica dos Santos Carvalho ; Márcia Sampaio Campos ; Sandra Márcia Habitante

Abstract

The search for effective protocols to reduce the incidence of root resorptions and allow the periodontal ligament repair is still challenging, given the unpredictable outcome of late tooth replantation. With the development of periodontal regenerative therapy, there is a new perspective for the treatment or prevention of these issues with tooth replantation. The present study has the purpose to evaluate, using radiographic, histological and immunohistochemistry analyses, the effect of the root surface conditioning with high power diode laser, associated or not to fibroblast growth factor 2 (FGF-2), in the incidence of external root resorption and in the periodontal repair process, in late replanted teeth. Seventy male Wistar rats will be used, being 10 animals for training and 60 for experimental purposes, from which the right central incisors will be extracted. After the extraction, the specimens will be randomly split in 5 groups (n=12). G1 and G2 will be the control groups (with no treatment for periodontal repair), and they will be respectively submitted to immediate and late replantation (60 minutes after the extraction). G3, G4 and G5 will be treated in advance of the late replant as follows: G3 - root surface conditioning with high power diode laser (810 nm, continuous mode, 600 µm fiber optics, 1.5 W, 30 s); G4 - root surface conditioning with the same parameters as G3, with further application of fibroblast growth factor 2 (FGF-2 gel) + hidroxipropilcellulose matrix on the whole root surface; and G5 - only with application of FGF-2 gel + hidroxipropilcellulose matrix as in G4. After 60 days of replantation, the animals will be euthanized, the specimens radiographed and processed for histological and immunohistochemistry analyses. (AU)