Physical training, fructose consumption and hepatic markers of metabolic syndrome in na experimental model

• Grant number: 10/52379-5
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2011
• End date: February 28, 2013
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Maria Alice Rostom de Mello
• Grantee: Maria Alice Rostom de Mello
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

Metabolic syndrome became a medical-social problem in both developed and developing countries and high dietary consumption of fructose seems to play an important role in this matter. Therefore, it is of interest the development of more effective procedures for the prevention and the treatment of this disease. As there are limitations in the studies with human beings, experimental models are important tools to address the issue. The present study was designed to analyze the effects of physical training on hepatic biomarkers of metabolic syndrome and on the aerobic capacity of rats fed a fructose rich diet. The study will be conducted in male Wistar rats that will be subjected to the lactate minimum test (Lac Min), for identification of the aerobic/anaerobic metabolic transition during swimming exercise and that, from 120 days until 180 days old on, will be separated into two groups, according to the diet: control (AIN 93 balanced diet) and fructose rich (60% fructose diet). Half of the animals of each group will be trained by a swimming protocol to be established at the beginning of the study and the other half will be kept sedentary. At the end of the study, the rats will be evaluated with respect to: aerobic capacity (Lac Min after training); glucose tolerance (oral glucose tolerance test), peripheral insulin sensivity (insulin tolerance test) and markers of non alcoholic fatty liver disease (serum ALT- aspartate aminotransferase and ALT- alanine aminotransferase activities). It will be also evaluated in the liver: total lipids and triglycerides concentrations; lipogenesis rates; biomarkers of the antioxidant system (catalase - CAT and superoxide dismutase - SOD activities) and of the lipid peroxidation (substances that reacts to the tiobarbituric acid - TBARs) as well as histology (hematoxylin/eosin) and in the serum: concentration of the cytokines TNF α, IL-1 ß, IFNγ (pro-inflammatory) and IL-4 and IL-10 (anti-inflammatory). (AU)