Physical Training, Fructose consumption and hepatic markers of metabolic syndrome in experimental model.

• Grant number: 09/15336-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2010
• Effective date (End): December 31, 2013
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Maria Alice Rostom de Mello
• Grantee: José Diego Botezelli
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

The metabolic syndrome has become a serious social-medical problem in developed and developing countries and excessive consumption of fructose in the diet appears to play a role in this matter. Therefore, it is of great interest to develop more effective interventions for prevention and treatment of this disease. The exercise is a procedure that deserves investigation. Because there are limitations with human research, it is necessary to use experimental models for better approach the investigation on the metabolic syndrome. This study aims to examine the effects of physical training on liver biomarkers of metabolic syndrome and aerobic capacity of rats fed on fructose rich diet. Male Wistar rats with 120 days of age, will be used. They will be separated into two groups according to diet: control (balanced diet AIN-93) and fructose (diet with 60% fructose) and submitted to the lactate minimum test (Lacmin), to identify the aerobic/anaerobic metabolic transition during swimming exercise. Half of the animals from each group will be trained by an exercise protocol be established at an early stage of this study was between 120 and 180 days and the other half will remain sedentary. In the end of experiment, it will be analyzed: aerobic capacity (Lacmin after training), glucose tolerance (oral glucose tolerance test), peripheral insulin sensitivity (insulin tolerance test); markers of Non Alcoholic Fatty Liver Disease (serum alanine aminotransferase - ALT, aspartate amionotransferse - AST and alkaline phosphatase - ALK). It will be also determined in the liver: total lipidis concentrations and triglycerides; rate lipogenesis; biomarkers of antioxidant defense system (enzymatic activity calalase - CAT and superoxide dismutase - SOD) and lipid peroxidation (substances which react to thiobarbituric acid - TBARS).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOTEZELLI, JOSE DIEGO; CAMBRI, LUCIELI TERESA; GHEZZI, ANA CAROLINA; DALIA, RODRIGO AUGUSTO; VOLTARELLI, FABRICIO AZEVEDO; ROSTOM DE MELLO, MARIA ALICE. Fructose-rich diet leads to reduced aerobic capacity and to liver injury in rats. LIPIDS IN HEALTH AND DISEASE, v. 11, JUN 19 2012. (09/15336-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BOTEZELLI, José Diego. Exercício físico, ingestão de frutose e marcadores da síndrome metabólica em modelo experimental. 2014. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.