Global gene expression in papillary thyroid carcinoma: search for diagnostic, prog...
| Grant number: | 10/18370-0 |
| Support type: | Regular Research Grants |
| Duration: | May 01, 2011 - October 31, 2013 |
| Field of knowledge: | Biological Sciences - Biochemistry - Molecular Biology |
| Principal Investigator: | Luiz Paulo Kowalski |
| Grantee: | Luiz Paulo Kowalski |
| Home Institution: | Hospital A C Camargo. Fundação Antonio Prudente (FAP). São Paulo , SP, Brazil |
| Assoc. researchers: | Silvia Regina Rogatto |
Abstract
Papillary thyroid carcinoma is one of the most frequent malignant endocrine neoplasia and increasing in prevalence in the last decades. The prognostic is usually favorable nevertheless death risk stratification is essential to determine treatment aggressiveness and the level of vigilance for the patient. Among the most important factors used to determine the risks of recurrence or death risk by the disease is extrathyroidal extension. After thyroidectomy, radioiodine therapy is frequently indicated, which helps the post-operative following and reduces the risk of death by disease. In this study, we will perform expression oligoarrays analysis on papillary thyroid carcinoma with or without extrathyroidal extension, the first one considered as more aggressive. The aim of this analysis is to identify essential factors of tumor progression from initial stages to more aggressive. The prevalence of common mutations in this type of neoplasia (in RAS and BRAF) will be evaluated according to extrathyroidal extension status. In addition, the importance of the participation of iodine incorporation pathways on recurrence after radioiodine therapy will be evaluated, using immunohistochemistry in a platform of tissue microarray (TMA). Through the integration of clinical and molecular data, algorithms aiming to predict disease recurrence will be elaborated. The markers and models established will be evaluated in independent sets of validation by transcriptional and protein expression assays. (AU)