Acquisition of a fluorescence correlation spectroscope for the measurement of fluorescence correlation and image capture by fluorescence lifetime

• Grant number: 09/54044-3
• Support Opportunities: Multi-user Equipment Program
• Start date: November 01, 2010
• End date: October 31, 2012
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Amando Siuiti Ito
• Grantee: Amando Siuiti Ito
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

This proposal is aimed at the acquisition of equipment needed in order to conduct experiments involving fluorescence correlation spectroscopy (FCS), which is a technique for the analysis of biomolecules at extremely low concentrations, with high spatial and temporal resolution. Spontaneous fluctuations in intensity, caused by small deviations in the thermal balance of the system, constitute the parameter of interest. The spectroscopic measurements can be combined with those of the lifetime of the fluorescence emissions, and the data can be treated in order to obtain images in the process known as fluorescence lifetime imaging microscopy (FLIM). The technique is employed in biophysics and biochemistry: in experiments designed to measure the diffusion coefficients, kinetic rate constants, molecular aggregation, polydispersity, and molecular mass; in studies of antigen-antibody interactions, receptor-ligand relationships, nucleic acid interactions, interactions among proteins, enzyme activity, and protein folding processes; and in experiments involving single molecules and molecular motors. Project participants include researchers at the USP Ribeirão Preto School of Philosophy, Science, and Literature, the USP Institute of Physics Institute, the USP São Carlos Institute of Physics, and the Federal University of São Paulo. Despite the growing use of FCS and FLIM in studies in the areas of biophysics, biochemistry, and materials sciences worldwide, there is no FCS or FLIM equipment in the state of São Paulo. The acquisition of such equipment for use in the multi-user modality will make it possible for innumerable researchers to employ the technique. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TOVANI, CAMILA BUSSOLA; VENTRICI DE SOUZA, JOAO FRANCISCO; CAVALLINI, THIAGO DE SOUZA; DEMETS, GREGOIRE JEAN-FRANCOIS; ITO, AMANDO; BARIONI, MARINA BERARDI; PAZIN, WALLANCE MOREIRA; DARBELLO ZANIQUELLI, MARIA ELISABETE. Comparison between cucurbiturils and beta-cyclodextrin interactions with cholesterol molecules present in Langmuir monolayers used as a biomembrane model. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 111, p. 398-406, NOV 1 2013. (11/00850-9, 09/54044-3)

ITO, AMANDO SIUITI; RODRIGUES, ANA PAULA; PAZIN, WALLANCE MOREIRA; BARIONI, MARINA BERARDI. Fluorescence depolarization analysis of thermal phase transition in DPPC and DMPG aqueous dispersions. Journal of Luminescence, v. 158, p. 153-159, FEB 2015. (09/54044-3)

BARIONI, MARINA BERARDI; RAMOS, ANA PAULA; DARBELLO ZANIQUELLI, MARIA ELISABETE; ACUNA, ALBERTO ULISES; ITO, AMANDO SIUITI. Miltefosine and BODIPY-labeled alkylphosphocholine with leishmanicidal activity: Aggregation properties and interaction with model membranes. Biophysical Chemistry, v. 196, p. 92-99, JAN 2015. (10/08652-9, 09/54044-3)