Flow cytometry for the Children’s Hospital and the Institute for Treatment of Childhood Cancer at the University of São Paulo School of Medicine (FM USP)

• Grant number: 09/53864-7
• Support Opportunities: Multi-user Equipment Program
• Start date: October 01, 2010
• End date: September 30, 2012
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Magda Maria Sales Carneiro-Sampaio
• Grantee: Magda Maria Sales Carneiro-Sampaio
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The Children’s Hospital (ICr) and its Service of Onco-Hematology, located at the Institute for Treatment of Childhood Cancer (ITACI), which are part of FM USP Clinics Hospital (HC), have in recent years actively sought to implement research activities, based on their significant cases of children and adolescents with complex and rare diseases, working in concert with the LIM-36, Laboratory for Medical Research 36, which is dedicated to studies in Clinical Pediatrics. With the support of two Thematic Projects funded by FAPESP (grant nos. 2002/05880-4 and 2008/58238-4), their research in the field of primary immunodeficiencies (PIDs) is well established, involving the Divisions of Pediatric Immunology and Pediatric Rheumatology, and focuses primarily on the study the autoimmune and inflammatory manifestations of PIDs. Recently, the ICr entered into a partnership with the HC Heart Institute to implement the functional investigation of the human thymus. The ICr also has a genetics clinic, which follows significant numbers of children with different monogenic defects, generating data that is also valuable for investigating the human immune response. The ITACI, for its part, has partnered with the Department of Immunology of the USP Institute of Biomedical Sciences to investigate the pathophysiology of dendritic cells for immunotherapy of neuroblastoma, a type of cancer that typically affects children and for which the Itaci is a referral center, receiving approximately 10% of all cases occurring in Brazil. Twenty years after the first bone marrow transplant procedure was performed at the ICr (27/10/1989), the São Paulo Center for Pediatric Hematopoietic Stem Cells Transplantation is being built at the Itaci, with funding from the state government. The Center will be launched in 2012, and will also receive patients with severe PIDs. Therefore, the deployment of a flow cytometry platform in LIM-36 would be of immense value in a number of areas: i) PIDs (emphasis on inflammatory diseases, autoimmune disorders, and genetic syndromes causing an impaired immune response); ii) pediatric oncology (emphasis in neuroblastoma and leucemia-60 new cases/year); and iii) cell transplantation in children with hematopoietic malignancies, PIDs and other diseases. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GRASSI, MARCILIA S.; JACOB, CRISTINA M. A.; KULIKOWSKI, LESLIE D.; PASTORINO, ANTONIO C.; DUTRA, ROBERTA L.; MIURA, NANA; JATENE, MARCELO B.; PEGLER, STEPHANIE P.; KIM, CHONG A.; CARNEIRO-SAMPAIO, MAGDA. Cardiopatias Congênitas como um Sinal de Alerta para o Diagnóstico da Deleção do 22q11.2. Arquivos Brasileiros de Cardiologia, v. 103, n. 5, p. 382-390, NOV 2014. (08/58238-4, 09/53105-9, 09/53864-7)

ZAGO, CLAUDIA AUGUSTA; ABE JACOB, CRISTINA MIUKI; DE ALBUQUERQUE DINIZ, EDNA MARIA; LOVISOLO, SILVANA MARIA; NOGUEIRA ZERBINI, MARIA CLAUDIA; DORNA, MAYRA; WATANABE, LETICIA; FERNANDES, JULIANA FOLLONI; ROCHA, VANDERSON; OLIVEIRA, JOAO BOSCO; et al. Autoimmune manifestations in SCID due to IL7R mutations: Omenn syndrome and cytopenias. HUMAN IMMUNOLOGY, v. 75, n. 7, p. 662-666, JUL 2014. (08/58238-4, 09/53864-7)

CERONI, J. R. M.; DUTRA, R. L.; HONJO, R. S.; LLERENA, JR., J. C.; ACOSTA, A. X.; MEDEIROS, P. F. V.; GALERA, M. F.; ZANARDO, E. A.; PIAZZON, F. B.; DIAS, A. T.; et al. A Multicentric Brazilian Investigative Study of Copy Number Variations in Patients with Congenital Anomalies and Intellectual Disability. SCIENTIFIC REPORTS, v. 8, SEP 6 2018. (09/53105-9, 09/53864-7, 14/11572-8, 08/58238-4, 12/25247-6)

QUINELLO, C.; SILVEIRA-LESSA, A. L.; CECCON, M. E. J. R.; CIANCIARULLO, M. A.; CARNEIRO-SAMPAIO, M.; PALMEIRA, P.. Phenotypic Differences in Leucocyte Populations among Healthy Preterm and Full-Term Newborns. Scandinavian Journal of Immunology, v. 80, n. 1, p. 57-70, JUL 2014. (09/54246-5, 09/53864-7)