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Acquisition of a microarray platform for the Diadema Campus of the Federal University of São Paulo

Grant number: 09/53955-2
Support type:Multi-user Equipment Program
Duration: December 01, 2010 - November 30, 2012
Field of knowledge:Biological Sciences - Genetics - Human and Medical Genetics
Principal researcher:Fabíola Freitas de Paula Lopes
Grantee:Fabíola Freitas de Paula Lopes
Home Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Type of equipment: Tipo de Equipamento Multiusuário não informado
Manufacturer: Fabricante não informado
Model: Modelo não informado


The project envisages acquisition of a microarray platform for the Diadema Campus of Universidade Federal de São Paulo (the Federal University of São Paulo). The microarray technology consists of utilization of a microarrangement in which DNA samples are fixed on spots for hybridization with a pool of mRNAs extracted from biological samples (targets) that were previously marked with fluorescent dyes and allows for simultaneous evaluation of the expression of thousands of genes in different tissues of a given organism. This approach can also be applied in studies to analyze DNA methylation, pharmacogenetis, cytogenetics, genotyping, mRNA expression, etc. Scientists chose the Agilent platform based on its better price and lower operational costs at the existing authorized dealer in Brazil (GE Healthcare Bio-Sciences Corp.) and above all, because of the flexibility of the system, which allows operators to use blades made by different manufacturers. Additional equipment necessary for the operating the platform will be acquired. A management plan will be created with the main objective of ensuring management, maintenance and multi-user access to the equipment, allowing for and facilitating usage of this equipment by researchers affiliated to this and other institutions. The management commission will be initially formed by researchers associated with this proposal and by the technicians assigned by the host-institution. And finally, there are several complementary projects, many of which at other institutions, which will also benefit from this request, allowing for future collaboration and greater scientific impact. In this manner, acquisition of the microarray platform will not only contribute to new scientific discoveries within associated and complementary projects, but will give a major boost to research from different areas that are part of this campus. (AU)

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