Infrastructure for metabolic, molecular biology, and cell biology analyses in projects related to functional and comparative genomics

• Grant number: 09/54203-4
• Support Opportunities: Multi-user Equipment Program
• Start date: September 01, 2010
• End date: August 31, 2014
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Gabriel Henrique Marroig Zambonato
• Grantee: Gabriel Henrique Marroig Zambonato
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The USP Biosciences Institute is a center of excellence in research, participating in cutting-edge projects, such as the Center for Human Genome Studies (funded by the FAPESP Centers for Research, Innovation, and Dissemination Program), FAPESP Thematic Projects, genome projects, projects funded by the FAPESP Program for Research on Bioenergy, and National Institute of Science and Technology projects. Currently, the Biosciences Institute is in a phase of expansion, having recently opened 16 new teaching positions and constructed a new building, with the objective of bringing together professors working in various specialties. The academic excellence acquired by the various groups at the Biosciences Institute can be strengthened by the establishment of a multi-user facility, tentatively designated the Laboratório Multiusuário de Análises Celulares e Moleculares (Lamcemol, Multi-User Laboratory for Cellular and Molecular Analyses). Therefore, the present project is aimed at equipping the Lamcemol, on the basis of the “facility” concept, with a high-resolution microtomograph (micro-CT) and a mass spectrometer (LTQ Orbitrap). The micro-CT (SkyScan 1076) combines the resolution of microscopes with the study of living systems through computed microtomography, allowing three-dimensional reconstructions, with a precision (pixel size) as fine as 7 microns, of organisms or entire structures, without the need for dissection. This makes it possible to obtain morphological data for cells, organs, and systems in a non-destructive manner. The other multi-user apparatus included in this proposal is a high-performance mass spectrometer, which allows the mass of molecules to be determined with great precision. The spectrometer has a number of applications, including those related to post-translational modifications, interactions among biomolecules, proteomics, and metabolic studies. The spectrometer requested features a combination of high resolution, precision in the determination of mass, and sensitivity (down to the subfemtomole level). The Lamcemol will be beneficial for various groups of the Biosciences Institute, because it will enable the execution of procedures including, but not limited to, the following: sequencing of DNA and proteins; identification of proteins that bind to biomolecules; analyses of proteinprotein interactions; proteomic analyses; and metabolomic analyses. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SIMON, MONIQUE NOUAILHETAS; MACHADO, FABIO ANDRADE; MARROIG, GABRIEL. High evolutionary constraints limited adaptive responses to past climate changes in toad skulls. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, v. 283, n. 1841, OCT 26 2016. (11/14295-7, 11/21674-4, 09/54203-4, 11/07584-2)

SIMON, MONIQUE NOUAILHETAS; MARROIG, GABRIEL. Landmark precision and reliability and accuracy of linear distances estimated by using 3D computed micro-tomography and the open-source TINA Manual Landmarking Tool software. Frontiers in Zoology, v. 12, JUN 3 2015. (09/54203-4, 11/14295-7, 11/07584-2)

SIMON, MONIQUE NOUAILHETAS; MARROIG, GABRIEL. Evolution of a complex phenotype with biphasic ontogeny: Contribution of development versus function and climatic variation to skull modularity in toads. ECOLOGY AND EVOLUTION, v. 7, n. 24, p. 10752-10769, DEC 2017. (09/54203-4, 11/14295-7, 11/07584-2)