Acquisition of a flow cytometer and liquid scintillation (beta radiation) counter for the phenotypic and functional analysis of immune system cells in experimental models of cancer

• Grant number: 09/53837-0
• Support Opportunities: Multi-user Equipment Program
• Start date: August 01, 2010
• End date: July 31, 2012
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: José Daniel Lopes
• Grantee: José Daniel Lopes
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• City of the host institution: São Paulo
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

This proposal aimed the acquisition of a flow cytometer and a microplate counter. The devices will be integrated into the equipment park of the Department of Microbiology, Immunology and Parasitology at the UNIFESP Paulista School of Medicine. This request is based on three associated projects and ten complementary projects. Among the associated projects are three FAPESP-funded projects (two Thematic Projects and one Young Investigators at Emerging Institutions Program project). Although addressing various topics in immunology, such as cancer, infections caused by fungi or parasites, autoimmunity and transplantation, all associated and complementary projects supporting this proposal have a common need to examine the phenotypic and functional characteristics of the cells, lymphoid or not, involved in each of those processes. In this context, methodological procedures such as cytometry and beta radiation quantitation become essential. (AU)