Acquisition of an in vivo microtomography scanner for use in the Laboratory for Characterization and Evaluation of Biological Responses

• Grant number: 09/54080-0
• Support Opportunities: Multi-user Equipment Program
• Start date: October 01, 2010
• End date: September 30, 2012
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Elcio Marcantonio Junior
• Grantee: Elcio Marcantonio Junior
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The objective of this proposal is the acquisition of a scanner for in vivo computed microtomography, to be used jointly by a network of researchers investigating tissue responses. The equipment is designed to visualize and perform qualitative and quantitative in vivo analyses of anatomical parameters in small animals, but can also be used to run biopsy analyses. Comparatively, in vivo microtomography has many advantages over ex vivo microtomography: 1-it allows longitudinal evaluation in the same animals; 2-it reduces the number of animals per experiment, because it allows the animals to be monitored, thereby avoiding the need to sacrifice animals at every time point to be analyzed, which has obvious ethical implications; 3-it allows physiological parameters, such as breathing, heart rate, temperature, and electrocardiographic data, to be monitored in real time, guaranteeing the maintenance of animals during the procedures and providing data for research in the area; 4-it is capable of image reconstruction in real time, allowing immediate analysis of data and increasing the number of analyses that can be made in a given period of time; 5-it has an X-ray energy potential of 120 kVp, allowing better results for images of implants, with a reduction in beam hardening artifacts. This equipment will be employed by a network of laboratories that engage in molecular biology, biomechanics, histological processing and image analysis, as well as the sectioning of hard tissues and metals. Because it can be employed in various fields of study, including medicine, veterinary sciences, dentistry, physiology and biomaterials development, there is a wide range of potential users. Therefore, the researchers affiliated with this proposal (associates and collaborators) work in various areas and at various universities within the State of São Paulo. The acquisition of this equipment is justified by the breadth of the team, the broad variety of applications, the lack of similar equipment in the state of São Paulo, and the quality of analyses that can be obtained. In addition, this equipment will contribute greatly to the development of research in the State of São Paulo and in Brazil as a whole. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ELBURKI, MUNA S.; ROSSA, CARLOS; GUIMARAES, MORGANA R.; GOODENOUGH, MARK; LEE, HSI-MING; CURYLOFO, FABIANA A.; ZHANG, YU; JOHNSON, FRANCIS; GOLUB, LORNE M.. A Novel Chemically Modified Curcumin Reduces Severity of Experimental Periodontal Disease in Rats: Initial Observations. Mediators of Inflammation, 2014. (10/19660-2, 12/15826-9, 09/54080-0)

ELBURKI, MUNA S.; ROSSA, CARLOS; GUIMARAES, MORGANA R.; GOODENOUGH, MARK; LEE, HSI-MING; CURYLOFO, FABIANA A.; ZHANG, YU; JOHNSON, FRANCIS; GOLUB, LORNE M.. A Novel Chemically Modified Curcumin Reduces Severity of Experimental Periodontal Disease in Rats: Initial Observations. Mediators of Inflammation, v. 2014, p. 10-pg., 2014-01-01. (10/19660-2, 12/15826-9, 09/54080-0)

ELBURKI, MUNA S.; ROSSA, JR., CARLOS; GUIMARES-STABILI, MORGANA R.; LEE, HSI-MING; CURYLOFO-ZOTTI, FABIANA A.; JOHNSON, FRANCIS; GOLUB, LORNE M.. A Chemically Modified Curcumin (CMC 2.24) Inhibits Nuclear Factor kappa B Activation and Inflammatory Bone Loss in Murine Models of LPS-Induced Experimental Periodontitis and Diabetes-Associated Natural Periodontitis. Inflammation, v. 40, n. 4, p. 1436-1449, AUG 2017. (10/19660-2, 12/15826-9, 10/20091-2, 09/54080-0)