Acquisition of two large-capacity devices (a Milliplex Analyzer Xponent 3 and accessories and a 7900 HT Fast Real Time PCR System) for research conductedat the University of São Paulo School of Dentistry and at other research institutes

• Grant number: 09/53848-1
• Support Opportunities: Multi-user Equipment Program
• Start date: November 01, 2010
• End date: October 31, 2012
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Carlos Ferreira dos Santos
• Grantee: Carlos Ferreira dos Santos
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The use of a multiplexing device, which can simultaneously analyze up to 100 proteins in a single sample, will resolve all the problems reported and will allow new studies to be designed. The real-time PCR equipment requested will be essential for the study of new drugs as well as applications such as gene expression and detection of single nucleotide polymorphisms. One update accessory requested in conjunction with the thermal cycler allows access to a powerful, easy to use tool that eliminates intensive pipetting steps and provides gene expression results to meet a high demand. The Bauru School of Dentistry currently has 76 researchers in two graduate programs, which involve the direct participation of 182 graduate students, as well as several undergraduate researchers and interns. These researchers will benefit from the acquisition of the multiplexing system and the real-time PCR equipment, as will researchers at other research centers, such as the Lauro de Souza Lima Institute (through Dr. Ana Paula Trombone) and the São Paulo State University Bauru Campus (through Dr. Sandra Lia do Amaral) and Araçatuba Campus (through Dr. Sandra Helena Penha de Oliveira). The acquisition of this equipment is of paramount importance for setting up the Laboratory of Immunology and Cell Biology and the Laboratory of Pharmacology, enabling projects in the clinical areas (dentistry, periodontics etc.), as well as in the areas of anatomy, pharmacology, immunology and microbiology, and will contribute to greater integration between researchers of this school and those of other centers. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

THAIS FRANCINI GARBIERI; DANIEL THOMAS BROZOSKI; THIAGO JOSÉ DIONÍSIO; CARLOS FERREIRA SANTOS; LUCIMARA TEIXEIRA DAS NEVES. Human DNA extraction from whole saliva that was fresh or stored for 3, 6 or 12 months using five different protocols. Journal of Applied Oral Science, v. 25, n. 2, p. 147-158, 2017-04-00. (09/53848-1, 11/22434-7)

SANTOS, CARLOS F.; MORANDINI, ANA C.; DIONISIO, THIAGO J.; FARIA, FLAVIO A.; LIMA, MARTA C.; FIGUEIREDO, CAIO M.; COLOMBINI-ISHIKIRIAMA, BELLA L.; SIPERT, CARLA R.; MACIEL, RUBENS P.; AKASHI, ANA P.; et al. Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue. PLoS One, v. 10, n. 8, AUG 5 2015. (13/16113-9, 05/60167-0, 09/53848-1, 09/15372-5, 10/01230-1)

SIPERT, CARLA RENATA; MORANDINI, ANA CAROLINA; DIONISIO, THIAGO JOSE; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; PENHA OLIVEIRA, SANDRA HELENA; CAMPANELLI, ANA PAULA; KUO, WINSTON PATRICK; SANTOS, CARLOS FERREIRA. In Vitro Regulation of CCL3 and CXCL12 by Bacterial By-products Is Dependent on Site of Origin of Human Oral Fibroblasts. JOURNAL OF ENDODONTICS, v. 40, n. 1, p. 95-100, JAN 2014. (07/00306-1, 05/60167-0, 09/53848-1)

SIPERT, CARLA R.; MORANDINI, ANA C.; DIONISIO, THIAGO J.; TRACHTENBERG, ALEXANDER J.; KUO, WINSTON P.; SANTOS, CARLOS F.. MicroRNA-146a and microRNA-155 show tissue-dependent expression in dental pulp, gingival and periodontal ligament fibroblasts in vitro. JOURNAL OF ORAL SCIENCE, v. 56, n. 2, p. 157-164, JUN 2014. (07/00306-1, 05/60167-0, 09/53848-1)