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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue

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Santos, Carlos F. [1] ; Morandini, Ana C. [1] ; Dionisio, Thiago J. [1] ; Faria, Flavio A. [1] ; Lima, Marta C. [1] ; Figueiredo, Caio M. [1] ; Colombini-Ishikiriama, Bella L. [1] ; Sipert, Carla R. [2] ; Maciel, Rubens P. [1] ; Akashi, Ana P. [1] ; Souza, Gabriela P. [1] ; Garlet, Gustavo P. [1] ; Rodini, Camila O. [1] ; Amaral, Sandra L. [3] ; Becari, Christiane [4] ; Salgado, Maria C. [4] ; Oliveira, Eduardo B. [4] ; Matus, Isaac [5] ; Didier, Daniela N. [5] ; Greene, Andrew S. [5]
Total Authors: 20
[1] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Sao Paulo - Brazil
[3] Sao Paulo State Univ, Fac Sci, Dept Phys Educ, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Sao Paulo - Brazil
[5] Med Coll Wisconsin, Dept Physiol, Milwaukee, WI 53226 - USA
Total Affiliations: 5
Document type: Journal article
Source: PLoS One; v. 10, n. 8 AUG 5 2015.
Web of Science Citations: 5

The initiation or progression of periodontitis might involve a local renin-angiotensin system (RAS) in periodontal tissue. The aim of this study was to further characterize the local RAS in human and rat periodontal tissues between healthy and periodontally-affected tissue. Components of the RAS were investigated using in vitro, ex vivo and in vivo experiments involving both human and Wistar rat periodontium. Although not upregulated when challenged with P. gingivalis-lipopolysaccharide, human gingival and periodontal ligament fibroblasts expressed RAS components. Likewise, healthy and inflamed human gingiva expressed RAS components, some of which were shown to be functional, yet no differences in expression were found between healthy and diseased gingiva. However, in inflamed tissue the immunoreactivity was greater for the AT(1)R compared to AT(2)R in fibroblasts. When compared to healthy tissue, ACE activity was increased in human gingiva from volunteers with gingivitis. Human-gingiva homogenates generated Ang II, Ang 1-9 and Ang 1-7 when incubated with precursors. In gingiva homogenates, Ang II formation from Ang I was nearly abolished only when captopril and chymostatin were combined. Ang 1-7 formation was significantly greater when human gingiva homogenates were incubated with chymostatin alone compared to incubation without any inhibitor, only captopril, or captopril and chymostatin. In rat gingiva, RAS components were also found; their expression was not different between healthy and experimentally induced periodontitis (EP) groups. However, renin inhibition (aliskiren) and an AT(1)R antagonist (losartan) significantly blocked EP-alveolar-bone loss in rats. Collectively, these data are consistent with the hypothesis that a local RAS system is not only present but is also functional in both human and rat periodontal tissue. Furthermore, blocking AT(1)R and renin can significantly prevent periodontal bone loss induced by EP in rats. (AU)

FAPESP's process: 13/16113-9 - Signaling by purinergic receptor P2X7 and histone deacetylases in the innate immune response of human periodontal fibroblasts: implications in the pathogenesis of periodontitis
Grantee:Ana Carolina Morandini Ramos
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 05/60167-0 - Expression of procollagen type 1, MIP-1± alpha and SDF-1 alpha by human fibroblasts stimulated pulp lipoteichoic acid from Streptococcus mutans
Grantee:Carlos Ferreira dos Santos
Support Opportunities: Regular Research Grants
FAPESP's process: 09/53848-1 - Acquisition of two large-capacity devices (a Milliplex Analyzer Xponent 3 and accessories and a 7900 HT Fast Real Time PCR System) for research conductedat the University of São Paulo School of Dentistry and at other research institutes
Grantee:Carlos Ferreira dos Santos
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 09/15372-5 - Interaction between the renin-angiotensin system and the vascular endothelial growth factor in the experimentally-induced periodontal disease in rats
Grantee:Daniel Thomas Brozoski
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 10/01230-1 - The role of TLR2 and TLR4 in the production of cytokines by human periodontal fibroblasts deficient of these receptors
Grantee:Carlos Ferreira dos Santos
Support Opportunities: Regular Research Grants