Gene and protein expression of MDM4, PI3KC2ß, KIF14, DDX59, CDH11 in Ewing sarcomas

• Grant number: 10/18065-3
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2011
• End date: June 30, 2013
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Isabela Werneck da Cunha
• Grantee: Isabela Werneck da Cunha
• Host Institution: A C Camargo Cancer Center. Fundação Antonio Prudente (FAP). São Paulo , SP, Brazil

Abstract

Ewing tumors are osseous and extraosseous neoplasias mainly found in children and teenagers. They are aggressive, usually presenting local recurrences and metastasis. The treatment response may be variable, and some patients may present favorable treatment outcome without exhibit eventual problems. Recent molecular cytogenetic studies from our group showed relevant genetic alterations at loci of genes, 1q32.1 (MDM4, PI3KC2ß, KIF14, DDX59) and 16q21 (CDH11), as important prognostic value for Ewing patient tumors. Thus, the present study aimed to validate those results evaluating the genes and proteins expression by qRT-PCR and immunohistochemistry. The future findings may help to recognize Ewing tumors with distinct biological behavior and treatment response. (AU)