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Effects of rock kinase inhibition in the invasive potential of Ewing Sarcoma cell lines

Grant number: 14/07118-0
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2014
Effective date (End): March 31, 2016
Field of knowledge:Health Sciences - Medicine
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Luiz Gonzaga Tone
Grantee:Gabriela Maciel Vieira
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Cancer is the leading cause of death by disease among children and adolescents for all regions of Brazil as well as in developed countries. Among them, the Ewing Sarcoma (ES) is the second most common bone tumor. Despite survival improvements of patients with localized disease, the incidence of metastasis remains very high, and about 30-40 % of patients with disease progression will die because of chemoresistance and high invasiveness of cells. Currently, ROCKs proteins has awakened an interest as a therapeutic strategy in multiple tumor types, due to its numerous functions related to cell migration, invasion, adhesion and proliferation, processes that are essential for tumor metastasis. Thus, we propose to evaluate the expression of ROCK1 and ROCK2 genes in samples of patients with Ewing's Sarcoma, and check the effects of their in vitro inhibition in SE cell lines of by synthetic drugs, emphasizing on proliferation, migration, invasion and metastasis. We also intend to evaluate the influence of ROCKs inhibition on resistance and sensitization to treatments currently available for this tumor type, conducting along in vivo experiments in immunodeficient mice to study the therapeutic potential of ROCK inhibition in relation to tumor dissemination. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROBERTO, GABRIELA MOLINARI; VIEIRA, GABRIELA MACIEL; ALBERICI DELSIN, LARA ELIS; SILVA, MARCELA DE OLIVEIRA; HAKIME, RODRIGO GUEDES; ENGEL, EDGARD EDUARD; SCRIDELI, CARLOS ALBERTO; TONE, LUIZ GONZAGA; BRASSESCO, MARIA SOL. MiR-708-5p is inversely associated with EWS/FLI1 Ewing sarcoma but does not represent a prognostic predictor. CANCER GENETICS, v. 230, p. 21-27, JAN 2019. Web of Science Citations: 2.
VIEIRA, GABRIELA MACIEL; ROBERTO, GABRIELA MOLINARI; LIRA, REGIA CAROLINE; ENGEL, EDGARD EDUARD; TONE, LUIZ GONZAGA; BRASSESCO, MARIA SOL. Prognostic value and functional role of ROCK2 in pediatric Ewing sarcoma. Oncology Letters, v. 15, n. 2, B, p. 2296-2304, FEB 2018. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
VIEIRA, Gabriela Maciel. Effects of ROCK kinases inhibition on the invasive potential of Ewing sarcoma cell lines. 2016. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto Ribeirão Preto.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.