Prophylactic and therapeutic strategies to control experimental autoimmune encephalomyelitis based on hsp65 immunoregulatory effect

• Grant number: 11/07528-5
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2011
• End date: December 31, 2013
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Alexandrina Sartori
• Grantee: Alexandrina Sartori
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

This investigation is based on the described ability of hsp65 to regulate autoimmune inflammatory diseases by inducing regulatory T cells. In this context, the major objective of this project is to evaluate the prophylactic and therapeutic effect of hsp65 on the clinical and immunological characteristics of experimental autoimmune encephalomyelitis (EAE). For this, C57BL/6 female will be injected with 3 DNAhsp65 doses or with a mixture of DNAhsp65 and the recombinant hsp65, before (prophylaxis) or after (therapy) EAE induction. Analysis of weight, clinical score, inflammation at the central nervous system and myelin specific immune response will allow to characterize the protective ability of these procedures. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONCALVES ZORZELLA-PEZAVENTO, SOFIA FERNANDA; CHIUSO-MINICUCCI, FERNANDA; DONEGA FRANCA, THAIS GRAZIELA; WATANABE ISHIKAWA, LARISSA LUMI; DA ROSA, LARISSA CAMARGO; COLAVITE, PRISCILA MARIA; MARQUES, CAMILA; VALERIO IKOMA, MAURA ROSANE; SILVA, CELIO LOPES; SARTORI, ALEXANDRINA. Downmodulation of peripheral MOG-specific immunity by pVAXhsp65 treatment during EAE does not reach the CNS. Journal of Neuroimmunology, v. 268, n. 1-2, p. 35-42, MAR 15 2014. (11/07528-5)

GONCALVES ZORZELLA-PEZAVENTO, SOFIA FERNANDA; CHIUSO-MINICUCCI, FERNANDA; DONEGA FRANCA, THAIS GRAZIELA; WATANABE ISHIKAWA, LARISSA LUMI; DA ROSA, LARISSA CAMARGO; COLAVITE, PRISCILA MARIA; BALBINO, BIANCA; MARQUES, CAMILA; VALERIO IKOMA, MAURA ROSANE; MASSON, ANA PAULA; et al. pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity. JOURNAL OF IMMUNOLOGY RESEARCH, v. 2017, p. 11-pg., 2017-01-01. (11/07528-5)

GONCALVES ZORZELLA-PEZAVENTO, SOFIA FERNANDA; CHIUSO-MINICUCCI, FERNANDA; DONEGA FRANCA, THAIS GRAZIELA; WATANABE ISHIKAWA, LARISSA LUMI; DA ROSA, LARISSA CAMARGO; COLAVITE, PRISCILA MARIA; BALBINO, BIANCA; MARQUES, CAMILA; VALERIO IKOMA, MAURA ROSANE; MASSON, ANA PAULA; et al. pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity. CLINICAL & DEVELOPMENTAL IMMUNOLOGY, 2017. (11/07528-5)