Identification of molecular markers attached to genes resistant to race 1 of oidium (Sphaerotheca fuliginea) and to Papaya ringspot virus, watermelon strain, in melon (Cucumis melo)

• Grant number: 02/09915-7
• Support Opportunities: Research Grants - Research Partnership for Technological Innovation - PITE
• Start date: July 01, 2003
• End date: June 30, 2006
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Luis Eduardo Aranha Camargo
• Grantee: Luis Eduardo Aranha Camargo
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Company: Sakata Seed Sudamerica Ltda
• City: Piracicaba

Abstract

Amongst the methodologies used for the identification of molecular markers linked to genes of interest (in this case, resistance to pathogens), the comparative analysis of almost isogenic strains, which differ from each other merely by the presence or absence of specific genes, is one of the most efficient. Two almost isogenic strains of melon, AF-426 (P1) and AF-2196 (P2), contrasting in the absence and presence, respectively, of alleles of resistance to Oidium and PRSV-W, are compared using Rapo (Random Amplified Polymorphic DNA) and AFLP (Amplified Fragment Length Polymorphism). These strains, in turn, are compared with AF-125 (P3), the donor strain of alleles of resistance. Markers present in the resistant converted strain P2 and in the donor strain P3 and absent in the susceptible recurrent strain P1 are considered potentially linked to the genes of resistance in question. The link between these marker candidates and the genes of resistance is confirmed using a population derived from the retrocrossing (P2 x P1) x P1, which is evaluated for resistance to two pathogens, in different experiments conducted in greenhouse conditions. There being a close link between the marker and the gene (s) of resistance, the ultimate objective will be to sequence the linked markers and transform them into Scar (Sequence Characterized Amplified Region) type markers, aiming for their use in programs of genetic improvement to resistance aided by markers. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TEIXEIRA‚ APM; CAMARGO‚ LEA. A molecular marker linked to the Prv1 gene that confers resistance to Papaya ringspot virus-type W in melon. PLANT BREEDING, v. 125, n. 2, p. 187-190, 2006. (02/09915-7)