Common bean response to the root knot nematode Meloidogyne incognita: QLT mapping, transcriptional profile and functional studies of candidate resistance genes

• Grant number: 20/02755-2
• Support Opportunities: Research Projects - Thematic Grants
• Start date: April 01, 2021
• End date: March 31, 2026
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Maria Lúcia Carneiro Vieira
• Grantee: Maria Lúcia Carneiro Vieira
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Pesquisadores principais: Antonio Augusto Franco Garcia
• Associated researchers: Alisson Fernando Chiorato ; Heqiang Huo ; Luciana Lasry Benchimol-Reis ; Luis Eduardo Aranha Camargo ; Saulo Fabrício da Silva Chaves
• Associated scholarship(s): 23/14857-2 - New phenotyping approaches for evaluating the common bean response to Meloidogyne incognita: use of quantitative PCR associated with nematological assessment indices., BP.PD; 22/10841-1 - Common bean breeding aiming at root-knot nematode resistance, BP.DR; 23/04476-1 - Phenotyping of common bean (Phaseolus vulgaris) infected with root-knot nematodes (Meloidogyne incognita) using spectral phenome, BP.IC; + associated scholarships 23/06169-9 - High-throughput phenotyping for the response of common bean populations to Meloidogyne incognita infection., BP.TT; 22/04061-3 - Genetic architecture of common bean resistance to the root knot nematode Meloidogyne incognita: F2 population construction, linkage map, and QTL mapping, BP.MS; 21/07118-3 - High-throughput phenotyping for the response of common bean populations to Meloidogyne incognita, BP.TT - associated scholarships

Abstract

Nematode attacks are considered among the major phytosanitary issues in common bean (Phaseolus vulgaris) crops. The root-knot nematodes of the genus Meloidogyne are possibly the most widely distributed pest, particularly M. incognita, one of the most damaging crop pathogens. The practices for nematode control usually involve infeasible field management. The chemical control is inefficient, expensive and raises environmental problems, demanding resistant cultivar development. Although the availability of new genetic and molecular approaches (SNPs for instance), a few has been done to understanding the mechanisms underlying the common bean response to root-knot nematodes. In this scenario, this project aims to move on with joint efforts of our part (IAC Common Bean Breeding Program and the ESALQ' Genetics Department), specially related to the identification of candidate genes and genomic regions involved in the common bean response to root-knot nematodes. This proposal comprises five subprojects, including: QTL mapping associated with resistance in the host; comparative transcriptional profiling of two contrasting genotypes; functional studies of candidate genes, via Agrobacterium transformation and gene editing; analysis of the interaction between the common bean - Fusarium oxysporum - M. incognita; and finally, the evaluation of pre-commercial breeding lines. Thus, the expected results may contribute to: understand the genetic architecture of the host resistance to the root-knot nematode, identify SNPs which allow the screening of resistant genotypes and marker assisted selection, reducing the necessity of the challenging phenotypic evaluation; move forward for unveiling the molecular mechanisms underlying the complex M. incognita - P. vulgaris interaction; identify genes related to the common bean response to root-knot nematodes, and develop and characterize cultivars in relation to the resistance. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GIORDANI, WILLIAN; GAMA, HENRIQUE CASTRO; CHIORATO, ALISSON FERNANDO; RODRIGUES MARQUES, JOAO PAULO; HUO, HEQIANG; BENCHIMOL-REIS, LUCIANA LASRY; ARANHA CAMARGO, LUIS EDUARDO; FRANCO GARCIA, ANTONIO AUGUSTO; CARNEIRO VIEIRA, MARIA LUCIA. Genetic mapping reveals complex architecture and candidate genes involved in common bean response to Meloidogyne incognita infection. PLANT GENOME, NOV 2021. (20/02755-2, 18/09069-7)

GIORDANI, WILLIAN; GAMA, HENRIQUE CASTRO; CHIORATO, ALISSON FERNANDO; FRANCO GARCIA, ANTONIO AUGUSTO; CARNEIRO VIEIRA, MARIA LUCIA. Genome-wide association studies dissect the genetic architecture of seed shape and size in common bean. G3-GENES, GENOMES, GENETICS, v. 12, n. 4, p. 16-pg., 2022-02-26. (18/09069-7, 20/02755-2, 14/06647-9)